Effects of air discharge on surface charges and cell walls of Fusarium oxysporum

被引:0
作者
Mengdie Liu
Hui Tang
Huiwen Jiang
Jie Li
Shoulei Yan
Qingzhang Wang
机构
[1] Huazhong Agricultural University,College of Food Science and Technology
[2] Aquatic Vegetable Preservation&Processing Technology Engineering Center of Hubei Province,undefined
[3] Engineering Research Center of Ministry of Education for Green Development of Aquatic Biological Industry in Yangtze River Economic Belt,undefined
来源
International Microbiology | 2021年 / 24卷
关键词
Air discharge; Surface charge; Cell wall; Chitin; Chitosan;
D O I
暂无
中图分类号
学科分类号
摘要
Air discharge showed significant inhibition on mycelial growth and spore germination of Fusarium oxysporum, one of the main spoilage fungi in post-harvest lotus roots which is an important economic aquatic vegetable in China. However, the antimicrobial mechanism of air discharge is not clear yet. In the present study, the effects of air discharge on F. oxysporum separated from post-harvest rotten lotus roots were characterized by analyzing surface charges, cell wall permeability, and changes in chitin and chitosan including surface morphology, functional groups, degree of deacetylation, crystallinity, and C/N ratio. After air discharge treatments, alkaline phosphatase leak assay revealed that cell wall permeability of F. oxysporum was magnified. What’s more, zeta potentials of F. oxysporum increased and negative charges on cell surfaces decreased. The ordered and compact molecular arrangements of chitin and chitosan in cell walls of F. oxysporum were reduced. The deacetylation degree of chitin and chitosan increased, and the C/N ratios of chitin and chitosan decreased. It was concluded from these results that air discharge caused the transformation in structures of chitin and chitosan, resulting in the exposure of positively charged amino groups and decrease of negative charges on cell surfaces which brought damage to the structure and function of F. oxysporum’s cell walls.
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页码:415 / 425
页数:10
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共 177 条
[1]  
Abdel-Aziz SM(2012)Extracellular metabolites produced by a novel strain Bacillus alvei NRC-14: 2. molasses-based growth for high production of N-acetylglucosamine Aust J Basic Appl Sci 6 251-261
[2]  
Mouafi FE(2015)Isolation and characterization of chitin from exoskeleton of pill-millipedes. Trends Biomater Artif Organs 29 155-159
[3]  
Keera AA(2019)Estimation of inactivation effects against Escherichia coli o157:h7 biofilm by different plasma-treated solutions and post-treatment storage Appl Phys Lett 114 073703.1-073703.5
[4]  
Ambarish CN(2005)A chitin synthase and its regulator protein are critical for chitosan production and growth of the fungal pathogen Cryptococcus neoformans Eukaryot Cell 4 1902-1912
[5]  
Sridhar KR(2006)Resistance to a polyquaternium-1 lens care solution and isoelectric points of Pseudomonas aeruginosa strains J Antimicrob Chemother 57 764-766
[6]  
An JY(2016)SEM characterization of anatomical variation in chitin organization in insect and arthropod cuticles Micron 82 74-85
[7]  
Yong HI(2013)Green synthesis approach: extraction of chitosan from fungus mycelia Crit Rev Biotechnol 33 379-403
[8]  
Kim HJ(1999)Influence of trolox derivative and N-acetylcysteine on surface charge density of erythrocytes in methanol intoxicated rats Environ Toxicol Pharmacol 8 15-21
[9]  
Park JY(2009)Synthesis, characterization and metal uptake capacity of a new carboxymethyl chitosan derivative Eur Polym J 45 199-210
[10]  
Lee SH(2002)Interaction of ozone and negative air ions to control micro-organisms J Appl Microbiol 93 144-148