Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsis brasiliensis

被引：0
作者：
Felipe H. A. Fernandes
Rayanne S. A. de Batist
Francinalva D. de Medeirosa
Fábio S. Santos
Ana C. D. Medeiros
机构：
[1] Universidade Estadual da Paraíba,Laboratório de Desenvolvimento e Ensaios de Medicamentos, Centro de Ciências Biológicas e da Saúde
[2] Universidade Federal da Paraíba,Laboratórios Unificados de Desenvolvimento e Ensaios de Medicamentos, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde
来源：
Revista Brasileira de Farmacognosia | 2015年 / 25卷
关键词：
Gallic acid; Chemical marker; Validation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The aim of this work was to develop and validate an analytical method for the identification of the chemical marker of Schinopsis brasiliensis Engl., Anacardiaceae. It would determine the total polyphenols and flavonoid content by spectrophotometric methodology in the dried extract of plant. The chromatographic profiles of S. brasiliensis were determined using HPLC-UV. The liquid chromatography method was conducted on a Phenomenex Gemini NX C18 column (250 × 4.6 mm, 5 μm). The mobile phase consisted of 0.05% orthophosphoric acid: methanol. The flow rate was 1 ml/min and effluents were monitored at 271 nm. The retention time for gallic acid was 8.5 min. The described method has the advantage of being both rapid and easy. Hence it can be applied for routine quality control analysis of herbal preparation containing S. brasiliensis.
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页码：208 / 211
页数：3
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