Modeling and observer design for recombinant Escherichia coli strain

被引：0
作者：
M. Nadri
I. Trezzani
H. Hammouri
P. Dhurjati
R. Longin
J. Lieto
机构：
[1] Université Claude Bernard Lyon I,L.A.G.E.P., UMR CNRS 5007
[2] University of Delaware,Department of Chemical Engineering
[3] Génomique Structurale,Plateau technique 5
来源：
Bioprocess and Biosystems Engineering | 2006年 / 28卷
关键词：
Modeling; Constant high-gain observer; Recombinant bacteria; Induction;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A mathematical model for recombinant bacteria which includes foreign protein production is developed. The experimental system consists of an Escherichia Coli strain and plasmid pIT34 containing genes for bioluminescence and production of a protein, β-galactosidase. This recombinant strain is constructed to facilitate on-line estimation and control in a complex bioprocess. Several batch experiments are designed and performed to validate the developed model. The design of a model structure, the identification of the model parameters and the estimation problem are three parts of a joint design problem. A nonlinear observer is designed and an experimental evaluation is performed on a batch fermentation process to estimate the substrate consumption.
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页码：217 / 225
页数：8
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