Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: A retrospective re-analysis

被引：2

作者：

Beissbarth J. ^{[1
]}

Binks M.J. ^{[1
]}

Marsh R.L. ^{[1
]}

Chang A.B. ^{[1
,2
,3
]}

Leach A.J. ^{[1
]}

Smith-Vaughan H.C. ^{[1
]}

机构：

[1] Menzies School of Health Research, Charles Darwin University, PO Box 41096, Casuarina, 0811, NT

[2] Department of Respiratory Medicine, Lady Cilento Children's Hospital, PO Box 3474, South Brisbane, 4101, QLD

[3] Institute of Health and Biomedical Innovation, Queensland University of Technology, GPO Box 2434, Brisbane, 4001, QLD

来源：

BMC Research Notes | / 11卷 / 1期

基金：

英国医学研究理事会; 美国国家科学基金会;

关键词：

Haemophilus haemolyticus (Hh); Nasopharynx; bronchoalveolar lavage; Nontypeable Haemophilus influenzae (NTHi);

D O I：

10.1186/s13104-018-3429-z

中图分类号：

学科分类号：

摘要：

Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens. © 2018 The Author(s).

引用

共 13 条

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[2]

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