Distribution of mecA among methicillin-resistant clinical staphylococcal strains isolated at hospitals in Naples, Italy

被引：26
作者：
E. Galdiero
G. Liguori
M. D'Isanto
N. Damiano
L. Sommese
机构：
[1] Dipto. di Fisiol. Gen. ed Ambientale, Facoltà di Scienze Biologiche, Universita Federico II, Napoli
[2] Dipartimento di Medicina Pubblica, Facoltà di Medicina/Chirurgia, Seconda Università di Napoli, 80138 Napoli
[3] Dipto. di Medicina Sperimentale, Facoltà di Medicina/Chirurgia, Seconda Università di Napoli, Napoli
来源：
European Journal of Epidemiology | 2003年 / 18卷 / 2期
关键词：
MecA; Methicillin-resistant staphylococci; Molecular epidemiolology;
D O I：
10.1023/A:1023067930211
中图分类号：
学科分类号：
摘要：
Two hundred and twenty strains of Staphylococcus isolated in Naples, Italy, were surveyed for the distribution of the mecA, the structural gene for penicillin-binding protein 2a, which is the genetic determinant for methicillin-resistance in staphylococci. Screening by a cloned mecA, revealed that of 220 strains, 43 were methicillin-resistant (19.5%) and 177 were methicillin-susceptible (80.5%). Among the 43 resistant strains 23 (53.5%) carried mecA in their genome and 20 (46.5%) did not carry mecA, in spite of their resistance to methicillin. Every group was submitted to the AP-PCR profiling. A quantitative analysis of the patterns divided strains into four different clusters for methicillin-resistant mecA-negative and two different clusters for methicillin-resistant mecA-positive with primer 1, while no clusters were noted with primer 7. We conclude that these clinical isolates from our area; were not found to belong to a single clone, although the predominance of four methicillin-resistant mecA-negative genotypes were noted.
引用
收藏
页码：139 / 145
页数：6
相关论文
共 33 条
  • [1] Al Ujayli B., Nafziger D.A., Saravolatz L., Pneumonia due to Staphylococcus aureus infection, Clin Chest Med, 16, pp. 111-120, (1995)
  • [2] Parker M.T., Phage typing of Staphylococcus aureus, Methods in Microbiology, 7 B, pp. 1-28, (1972)
  • [3] Maslow J.N., Mulligan M.E., Arbeit R.D., Molecular epidemiology: The application of contemporary techniques to typing of bacteria, Clin Infect Dis, 17, pp. 153-162, (1993)
  • [4] Tenover F.C., Arbeit R., Archer G., Et al., Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus, J Clin Microbiol, 32, pp. 407-415, (1994)
  • [5] Fagon J.Y., Chastre J., Vuagnat A., Et al., Nosocomial pneumonia and mortality among patients in intensive care units, JAMA, 275, pp. 866-869, (1996)
  • [6] Rello J., Torres A., Riccart M., Et al., Ventilator-associated pneumonia by Staphylococcus aureus. Comparison of methicillin-cresistant and methicillin-sensitive episodes, Am J Respir Crit Care Med, 150, pp. 1545-1549, (1994)
  • [7] Hackbarth C.J., Chambers H.F., Methicillin-resistant staphylococci: Genetics and mechanisms of resistance, Antimicrob Agents Chemother, 33, pp. 991-994, (1989)
  • [8] Suzuki E., Hiramatsu K., Yokota T., Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution, Antimicrob Agents Chemother, 36, pp. 429-434, (1992)
  • [9] Ryffel C., Tesch W., Birch-Machin I., Et al., Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis, Gene, 94, pp. 137-138, (1990)
  • [10] Berger-Bachi B., Barberis-Maino L., Strassle A., Et al., FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: Molecular cloning and characterization, Mol Gen Genet, 219, pp. 263-269, (1989)
1234