Determination of human urinary kanamycin in one step using urea-enhanced surface plasmon resonance light-scattering of gold nanoparticles

被引：0
作者：
Xinyi Wang
Mingjian Zou
Xiao Xu
Rong Lei
Kean Li
Na Li
机构：
[1] Peking University,The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering
[2] Shenyang Agricultural University,College of Sciences
来源：
Analytical and Bioanalytical Chemistry | 2009年 / 395卷
关键词：
Kanamycin; Plasmon resonance light-scattering; Gold nanoparticles; Urea enhancement;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The purpose of this study was to establish a simple, sensitive analytical method for kanamycin (KANA) in human urine. Enhancement of the plasmon resonance light-scattering (PRLS) of gold nanoparticles (AuNPs) by KANA provided the basis for this analytical method. At pH 6.7, KANA induced AuNPs aggregation with enhanced PRLS. The PRLS of the AuNPs–KANA system was further enhanced by addition of urea. The linear range and detection limit for KANA were from 20–800 nmol L−1 and 2 nmol L−1, respectively. Potential interfering substances present in urine had a negligible effect on the determination, thus preliminary sample separations were not necessary. Recovery of KANA from spiked human urine was 94–104%. This simple, sensitive method, using urea to enhance the PRLS of the AuNPs–KANA system, may provide a new approach for determination of compounds rich in OH groups.
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页码：2397 / 2403
页数：6
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  • [1] Nehl CL(2008)Shape-dependent plasmon resonances of gold nanoparticles J Mater Chem 18 2415-2419
  • [2] Hafner JH(2008)Photochemical and analytical applications of gold nanoparticles and nanorods utilizing surface-plasmon resonance Anal Bioanal Chem 391 2411-2421
  • [3] Uechi I(2008)The use of gold nanoparticles in diagnostics and detection Chem Soc Rev 37 2028-2045
  • [4] Yamada S(2008)Biological applications of gold nanoparticles Chem Soc Rev 37 1896-1908
  • [5] Wilson R(2008)Preparation of nanochain and nanosphere by self-assembly of gold nanoparticles Appl Phys Lett 92 3-551
  • [6] Sperling RA(2008)Colorimetric response of peptide-functionalized gold nanoparticles to metal ions Small 4 548-565
  • [7] Fang C(2002)Colorimetric detection of thiol-containing amino acids using gold nanoparticles (vol 127, pg 462, 2002) Analyst 127 565-169
  • [8] Slocik JM(2007)Telomere DNA conformation change induced aggregation of gold nanoparticles as detected by plasmon resonance light scattering technique Anal Chim Acta 604 165-116
  • [9] Zhang FX(2006)Resonance Rayleigh-scattering method for the determination of proteins with gold nanoparticle probe Anal Biochem 353 108-8430
  • [10] Huang CZ(2008)One-step label-free optical genosensing system for sequence-specific DNA related to the human immunodeficiency virus based on the measurements of light scattering signals of gold nanorods Anal Chem 80 8424-176
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