Cytotoxicity and single-dose oral toxicity testing for rice bran supercritical CO2 extract

被引：7
作者：
Choi J.-S. [1 ]
Moon W.-S. [2 ]
Moon J.-N. [2 ]
Cheon E.J. [3 ]
Son S.W. [4 ]
Kim J.-W. [5 ]
Kim M.-R. [3 ]
机构：
[1] RIS Center, IACF, Silla University, Sasang-gu, Busan
[2] Department of RandD, ECOMINE Co., Ltd, Nam-gu, Busan
[3] Department of Bio-Food Materials, Silla University, Sasang-gu, Busan
[4] Department of Dermatology, Korea University Ansan Hospital, Ansan
[5] Department of Veterinary Medicine, Kyungpook National University, Daegu
来源：
Toxicology and Environmental Health Sciences | 2013年 / 5卷 / 4期
关键词：
Cytotoxicity; Oral acute toxicity test; Rat; RAW; 264.7; cells; Rice bran supercritical CO2 extract (RB-SCE);
D O I：
10.1007/s13530-013-0175-4
中图分类号：
学科分类号：
摘要：
The aim of this study was to evaluate the toxicity of rice bran supercritical CO2 extract (RB-SCE) in vitro and in vivo. A cytotoxicity assay revealed no inhibitory effects on cell viability in a mouse leukemic monocyte macrophage cell line (RAW 264.7) after 24-h incubation with 0.16 or 0.32 μg/mL of RB-SCE. RB-SCE was administered to male and female Sprague-Dawley rats at 2,500, 5,000, or 10,000 mg/kg to evaluate acute oral toxicity. Mortality, clinical symptoms, and body weight changes were monitored for two weeks. Animals were then euthanized and necropsies performed. Clinical symptoms included soft stool, diarrhea, and soiled perineal region. No significant changes in body weight were observed in treatment groups compared to the control. In addition, necropsy revealed no abnormal finding at any dosage. The approximate lethal dose (ALD) of RB-SCE was estimated at >10,000 mg/kg for both sexes and orally administered RBSCE was deemed safe. © 2013 Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.
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收藏
页码：215 / 220
页数：5
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