Limitations in the diffusion of substrates into the flocs will condition cell metabolic behaviour, having obvious consequences on growth and product formation. Polymeric additives have been used aiming the reduction of those limitations. The knowledge of the concentration profiles and metabolic fluxes of glucose and oxygen inside the flocs would bring valuable information about the␣conditions under which a fermentation should run. Direct measurement of such profiles is rather difficult but their simulation has been performed and is presented in this work.