Expression of Apoptotic Epithelial Cells Within Lamina Propria Beneath the Basement Membrane Triggers Dextran Sulfate Sodium-Induced Colitis

被引：0
作者：
Kazuko Shichijo
Makoto Ihara
Mohammed Shawkat Razzaque
Mutsumi Matsuu-Matsuyama
Toshiyuki Nakayama
Masahiro Nakashima
Ichiro Sekine
机构：
[1] Nagasaki University Graduate School of Biomedical Sciences,Division of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute
[2] Nagasaki University Graduate School of Biomedical Sciences,Radiation Biophysics, Atomic Bomb Disease Institute
[3] Nagasaki University Graduate School of Biomedical Sciences,Department of Pathology II
[4] Nagasaki University Graduate School of Biomedical Sciences,Tissue and Histopathology Section, Atomic Bomb Disease Institute
来源：
Digestive Diseases and Sciences | 2008年 / 53卷
关键词：
Nuclear dust; Apoptosis; Bloody stools; Dextran sulfate sodium; Ulcerative colitis;
D O I：
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学科分类号：
摘要：
We postulated that nuclear dust within the lamina propria beneath the basement membrane of the epithelium in colonic mucosa is a form of apoptotic epithelial cells and that its expression triggers dextran sulfate sodium-induced colitis. The aim was to determine the origin of nuclear dust and to explore the correlation between nuclear dust expression and clinicopathologic parameters of colitis. Rats were treated with 3% dextran sulfate sodium. Cells showing double positive staining with cytokeratin and TdT-mediated uUTP-biotin nick-end labeling technique were apoptotic cells derived from epithelial cells. Nuclear dust expression on day 5 correlated with bloody stools and a decrease of mitotic colonic cells just before ulceration. Examination of cultures under light and fluorescent microscopy showed that dextran sulfate sodium caused early apoptosis and late apoptosis or necrosis. Our results suggest that intervetions directed toward the apoptotic process may be benifical in the treatment of ulcerative colitis.
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页码：2443 / 2451
页数：8
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