Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice

被引：0
作者：
Vijendra Prabhu
Bola Sadashiva Satish Rao
Anuradha Calicut Kini Rao
Keerthana Prasad
Krishna Kishore Mahato
机构：
[1] Manipal Academy of Higher Education,Department of Biophysics, Manipal School of Life Sciences
[2] Manipal Academy of Higher Education,Department of Biotechnology, Manipal Institute of Technology
[3] Manipal Academy of Higher Education,Department of Radiation Biology and Toxicology, Manipal School of Life Sciences
[4] Manipal Academy of Higher Education,Directorate of Research
[5] Manipal Academy of Higher Education,Department of Pathology, Kasturba Medical College
[6] Yenepoya Medical College,Department of Pathology
[7] Yenepoya (a Deemed to be University),Manipal School of Information Sciences
[8] Manipal Academy of Higher Education,undefined
来源：
Lasers in Medical Science | 2022年 / 37卷
关键词：
Excisional wounds; Wound repair; Photobiomodulation; Proliferating cell nuclear antigen; Ki67; Collagen deposition;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm2 when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm2 treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.
引用
收藏
页码：171 / 180
页数：9
相关论文
共 219 条
[1]  
Otterco AN(2018)Photobiomodulation mechanisms in the kinetics of the wound healing process in rats J Photochem Photobiol B 183 22-29
[2]  
Andrade AL(2011)Is the Ki-67 labelling index ready for clinical use? Ann Oncol 22 500-502
[3]  
Brassolatti P(1993)PCNA-binding to DNA at the G1/S transition in proliferating cells of the developing cerebral wall J Neurocytol 22 1096-1102
[4]  
Pinto KNZ(2014)Low-level laser irradiation stimulates tenocyte proliferation in association with increased NO synthesis and upregulation of PCNA and cyclins Lasers Med Sci 29 1377-1384
[5]  
Araujo HSS(2018)Photobiomodulation: lasers vs. light emitting diodes? Photochem Photobiol Sci 17 1003-1017
[6]  
Parizotto NA(2005)Light is light Photomed Laser Surg 23 159-160
[7]  
Jonat W(2003)Comparison of the photostimulatory effects of visible He-Ne and infrared Ga-As lasers on healing impaired diabetic rat wounds Lasers Surg Med 33 344-351
[8]  
Arnold N(2017)The JAK/STAT signaling pathway and photobiomodulation in chronic wound healing Cytokine Growth Factor Rev 38 73-79
[9]  
Takahashi T(1997)Helium-neon laser irradiation at fluences of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength Lasers Surg Med 20 340-345
[10]  
Caviness VS(1996)Low energy laser irradiation fails to modulate the inflammatory function of human monocytes and endothelial cells Lasers Surg Med 19 207-215
12345678910