Serological and molecular identification of Turnip mosaic virus in some wild plants in Iran

被引:5
作者
Valouzi H. [1 ]
Golnaraghi A. [2 ]
Abedini-Aminabad L. [1 ]
Diyanat M. [3 ]
机构
[1] Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University (IAU), P.O. Box 14515-775, Tehran
[2] Department of Plant Protection, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University (IAU), P.O. Box 14515-775, Tehran
[3] Department of Weed Science, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University (IAU), P.O. Box 14515-775, Tehran
关键词
Arctium lappa; Potyvirus universal primers; Sequence analysis; Serological assays; Sinapis arvensis; Turnip mosaic virus;
D O I
10.1007/s13314-016-0225-2
中图分类号
学科分类号
摘要
Forty leaf samples were collected from symptomatic wild plants grown in natural ecosystems in northeast of Tehran. These plant samples were tested by enzyme-linked immunosorbent assay (ELISA) using broad-spectrum potyvirus antibodies. Turnip mosaic virus (TuMV) was identified in four ELISA-positive samples by sequencing of their 3′ end of genomes and subsequently by specific ELISA tests. This study reports Arctium lappa and Sinapis arvensis as new hosts for TuMV in Iran. The results also seem to indicate the possible presence of a new genogroup of the virus. © 2016, Australasian Plant Pathology Society Inc.
引用
收藏
相关论文
共 16 条
[1]  
De Fatima Rosas-Cardenas F., Duran-Figueroa N., Vielle-Calzada J.P., Cruz-Hernandez A., Marsch-Martinez N., de Folter S., A simple and efficient method for isolating small RNAs from different plant species, Plant Methods, 7, (2011)
[2]  
Farzadfar S., Pourrahim R., Characterization of Turnip mosaic virus from the Asian-BR population in Iran, J Phytopathol, 162, pp. 824-828, (2014)
[3]  
Farzadfar S., Ohshima K., Pourrahim R., Golnaraghi A.R., Jalali S., Ahoonmanesh A., Occurrence of Turnip mosaic virus on ornamental crops in Iran, Plant Pathol, 54, (2005)
[4]  
Farzadfar S., Tomitaka Y., Ikematsu M., Golnaraghi A.R., Pourrahim R., Ohshima K., Molecular characterization of Turnip mosaic virus isolates from Brassicaceae weeds, Eur J Plant Pathol, 124, pp. 45-55, (2009)
[5]  
Nayudu M.V., Plant Viruses, (2008)
[6]  
Nguyen H.D., Tomitaka Y., Ho S.Y.W., Duchene S., Vetten H.J., Et al., Turnip mosaic Potyvirus probably first spread to Eurasian brassica crops from wild orchids about 1000 years ago, PLoS One, 8, (2013)
[7]  
Ohshima K., Yamaguchi Y., Hirota R., Hamamoto T., Tomimura K., Tan Z., Et al., Molecular evolution of Turnip mosaic virus
[8]  
evidence of host adaptation, genetic recombination and geographical spread, J Gen Virol, 83, pp. 1511-1521, (2002)
[9]  
Pappu S.S., Brand R., Pappu H.R., Rybicki E.P., Gough K.H., Frenkel M.J., Niblett C.L., A polymerase chain reaction method adapted for selective amplification and cloning of 3′ sequences of potyviral genomes: application to dasheen mosaic virus, J Virol Methods, 41, pp. 9-20, (1993)
[10]  
Shahraeen N., Farzadfar S., Lesemann D.E., Incidence of viruses infecting winter oilseed rape (Brassica napus ssp. oleifera) in Iran, J Phytopathol, 151, pp. 614-616, (2003)