Rapid in vitro propagation of Saussurea lappa, an endangered medicinal plant, through multiple shoot cultures

被引：10
作者：
Sudhakar Johnson T. [1 ]
Badari Narayan S. [1 ]
Narayana D.B.A. [1 ]
机构：
[1] Plant Biotechnology Laboratory, Dabur Research Foundation, New, Delhi, 110 002, 8/3, Asaf Ali Road
来源：
In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 2期
关键词：
Endangered medicinal plant; Ex situ conservation; Micropropagation; Multiple shoots; Saussurea lappa; Thidiazuron;
D O I：
10.1007/s11627-997-0010-1
中图分类号：
学科分类号：
摘要：
Rapid micropropagation of Saussurea lappa C.B. Clarke, an endangered medicinal plant endemic in the valley of Kashmin and western Himalayas of northern India, was achieved by culturing shoot tips (0.5-1 cm) of 2-wk-old seedlings on Murashige and Skoog's medium (MS) supplemented with thidiazuron (TDZ, 0.45 μM). Although callus-free multiple shoots were obtained both on N6-benzyladenine- (BA) and TDZ-containing media, TDZ was most effective (90%) in inducing multiple shoots. Shoot tips containing proliferative buds were divided into equal halves and subcultured on MS liquid medium containing 0.225 μM TDZ for further multiplication and elongation. Multiplication of induced shoot buds was more effective when cultured in liquid medium than on agar-solidified medium. Shoots (8-10 cm) developed were rooted in MS medium containing naphthaleneacetic acid (NAA, 1.07 μM). Micropropagated plantlets were successfully transferred to soil after hardening.
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页码：128 / 130
页数：2
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