Thermostable proteins as probe for the design of advanced fluorescence biosensors

被引：6

作者：

De Champdoré M. ^{[1
]}

Staiano M. ^{[1
]}

Aurilia V. ^{[1
]}

Stepanenko O.V. ^{[1
,2
]}

Parracino A. ^{[1
]}

Rossi M. ^{[1
]}

D'Auria S. ^{[1
]}

机构：

[1] Institute of Protein Biochemistry, Italian National Research Council, 80131 Naples

[2] Institute of Cytology, Russian Academy of Science

来源：

Reviews in Environmental Science and Bio/Technology | 2006年 / 5卷 / 2-3期

关键词：

Biosensors; Fluorescence; Glucose; Proteins; Thermophilic Organisms;

D O I：

10.1007/s11157-006-0009-9

中图分类号：

学科分类号：

摘要：

In this review we explore the advantages deriving from the use of either enzymes or sugar binding proteins isolated from thermophilic organisms to develop stable fluorescence biosensors. We report on a novel approach to address the consumption of the analyte by enzyme-based biosensors, namely the utilization of apo-enzymes as non-active forms of proteins which are still able to bind the ligand but cannot transform it into product. We also report recent studies in which the fluorescence labeling of a naturally thermostable binding protein allows a quantitative determination of glucose. © Springer Science+Business Media B.V. 2006.

引用

页码：233 / 242

页数：9

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