Expression and export: recombinant protein production systems for Aspergillus

被引：0
作者：
André Fleißner
Petra Dersch
机构：
[1] Institute of Genetics,Department of Molecular Infection Biology
[2] Technische Universität Braunschweig,undefined
[3] Helmholtz Centre for Infection Research,undefined
[4] Helmholtz-Zentrum für Infektionsforschung,undefined
来源：
Applied Microbiology and Biotechnology | 2010年 / 87卷
关键词：
Gene expression; Inducible promoters; Cloning vectors; Protein production; Secretion systems;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Several Aspergillus species, in particular Aspergillus niger and Aspergillus oryzae, are widely used as protein production hosts in various biotechnological applications. In order to improve the expression and secretion of recombinant proteins in these filamentous fungi, several novel genetic engineering strategies have been developed in recent years. This review describes state-of-the-art genetic manipulation technologies used for strain improvement, as well as recent advances in designing the most appropriate engineering strategy for a particular protein production process. Furthermore, current developments in identifying bottlenecks in the protein production and secretion pathways are described and novel approaches to overcome these limitations are introduced. An appropriate combination of expression vectors and optimized host strains will provide cell factories customized for each production process and expand the great potential of Aspergilli as biotechnology workhorses to more complex multi-step industrial applications.
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页码：1255 / 1270
页数：15
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