RAS-dependence of nerve growth factor-induced inhibition of proliferation of PC12 cells

被引：0
作者：
Katalin Kiss
B. Bartek
Nóra Nusser
J. Szeberényi
机构：
[1] University Medical School of Pécs,Department of Biology
来源：
Acta Biologica Hungarica | 1998年 / 49卷 / 1期
关键词：
PC12 cells; nerve growth factor; Ras proteins; Ha-Ras Asn17 mutant; proliferation;
D O I：
10.1007/BF03542981
中图分类号：
学科分类号：
摘要：
The PC12 rat phaeochromocytoma cell line is the most frequently used model system to study neuronal differentiation: nerve growth factor (NGF) inhibits proliferation and causes easily scorable neurite formation in these cells. The central role of the monomeric guanine nucleotide binding Ras proteins in the pathway of NGF-induced differentiation is well documented. However, the possible involvement of Ras proteins in signal transduction processes mediating NGF-induced inhibition of proliferation has not been analyzed. To address this problem we studied PC12 subclones expressing a dominant inhibitory mutant Ras protein (Ha-Ras Asnl7). Our results indicate that the cell cycle arrest characteristic of NGF-treated PC12 cells is mediated by a Ras-dependent signaling mechanism.
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页码：97 / 102
页数：5
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