Yellow Laccase-Mediated Lignin Degradation of Ricinus communis: A Future Agricultural Biomass for Biofuel Production

被引：9
作者：
Mukhopadhyay M. [1 ]
Banerjee R. [1 ]
机构：
[1] Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur
来源：
Agricultural Research | 2015年 / 4卷 / 03期
关键词：
FTIR; GC–MS; Genetic algorithm; Response surface methodology; Scanning electron microscopy; Yellow laccase;
D O I：
10.1007/s40003-015-0175-5
中图分类号：
学科分类号：
摘要：
Agricultural biomass left to decay in the fields and forests has a huge possibility to serve as a low-cost feedstock for production of bioethanol and other value added products. In the Indian subcontinent, Ricinus communis, growing in parched conditions contributes a major biomass containing 44.4 % cellulose, 22.8 % hemicelluloses and 19.8 % lignin. In the present study, response surface methodology based on central composite design and genetic algorithm has been used to explore the effects of pH, temperature, solid-to-liquid ratio (w/v), enzyme activity, and incubation time on enzymatic depolymerization of R. communis. The maximum delignification obtained was 86.7 %. Delignified biomass was characterized by Fourier transformed infrared spectroscopy and scanning electron microscopy. Lignin degradation products were identified by gas chromatography—mass spectroscopy. Cinnamic acid, p-coumaric acid (4-hydroxycinnamic acid), and ferulic acid (4-hydroxy-3-methoxycinnamic acid) were detected in the extract degraded by yellow laccase. © 2015, NAAS (National Academy of Agricultural Sciences).
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收藏
页码：309 / 318
页数：9
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