Vitamin C regulates the production of reactive oxygen species through Wnt10b signaling in the gill of zebrafish

被引：0
作者：
Dongwu Liu
Yaqi Gu
Hairui Yu
机构：
[1] Jiangnan University,State Key Laboratory of Food Science and Technology
[2] Shandong University of Technology,School of Agricultural Engineering and Food Science
[3] Shandong University of Technology,School of Life Sciences
[4] Weifang University,College of Biological and Agricultural Engineering, Weifang Bioengineering Technology Research Center
来源：
Fish Physiology and Biochemistry | 2021年 / 47卷
关键词：
Vitamin C; Wnt10b; β-catenin; Reactive oxygen species; Zebrafish;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, the mechanism that vitamin C (VC) regulates the production of reactive oxygen species (ROS) through Wnt10b signaling was investigated in the gill of zebrafish (Danio rerio). The results showed that 0.5 and 1.0 g/kg VC diets induced the gene expression of Wnt10b, β-catenin, SOD, CAT, and GSH-PX in gill. In addition, VC decreased the levels of H2O2, O2·− and ·OH, whereas the activities of SOD, CAT, and GSH-PX were increased by VC in the gill of zebrafish. To evaluate the role of Wnt10b in regulating oxidative stress, Wnt10b RNA was further interfered and the gene expression and activities of antioxidant enzymes were detected in gill. The result of Wnt10b RNA interference showed that Wnt10b signaling played a key role in regulating the gene expression of SOD, CAT, and GSH-PX. In all, VC may regulate the production of ROS through Wnt10b signaling in the gill of zebrafish (Danio rerio).
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页码：1271 / 1282
页数：11
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