Osteoprotective effects of Fructus Ligustri Lucidi aqueous extract in aged ovariectomized rats

被引：31
作者：
Ko C.H. [1 ]
Siu W.S. [1 ]
Lau C.P. [1 ]
Lau C.B.S. [1 ]
Fung K.P. [1 ,2 ]
Leung P.C. [1 ]
机构：
[1] Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin
[2] School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin
来源：
Chinese Medicine | / 5卷 / 1期
关键词：
Bone Mineral Density; Raloxifene; Adipogenic Differentiation; Bone Mineral Density Loss; Trabecular Bone Mineral Density;
D O I：
10.1186/1749-8546-5-39
中图分类号：
学科分类号：
摘要：
Background: Fructus Ligustri Lucidi (FLL) is a commonly used herb for treating bone disorders in Chinese medicine. The present study investigates the anti-osteoporotic activity of FLL aqueous extract in the model of postmenopausal bone loss in aged ovariectomized (OVX) female rats.Methods: After eight weeks of treatment of FLL or water, the lumbar spine was scanned by peripheral quantitative computed tomography (pQCT). Effects of FLL water extract on osteogenic and adipogenic differentiations in rat mesenchymal stem cells (MSCs) were assessed by biochemical methods and staining.Results: FLL aqueous extract significantly inhibited bone mineral density (BMD) loss in total, trabecular and cortical bones without affecting body weight and uterus wet weight. FLL extract significantly promoted osteogenesis and suppressed adipogenesis in MSCs as indicated by the elevated alkaline phosphatase activity, calcium deposition levels and decreased adipocyte number in a dose-dependent manner without cytotoxic effects. Real-time PCR analysis revealed significant increase of osteoprotegerin (OPG)-to-receptor activator for nuclear factor-κB ligand (RANKL) mRNA, indicating a decrease in osteoclastogenesis.Conclusion: The present study demonstrates the osteoprotective effects of FLL aqueous extract on aged OVX rats, stimulation of osteogenesis, inhibition of adipogenesis and osteoclastogenesis in MSCs. © 2010 Ko et al; licensee BioMed Central Ltd.
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共 25 条
[1]  
Li M.L., Progress in the study on Fructus Ligustri Lucidi, Zhongguo Zhong Yao Za Zhi, 19, 8, pp. 504-506, (1994)
[2]  
Sun Y., Lee S.M., Wong Y.M., Lau C.P., Shaw P.C., Qin L., Leung P.C., Fung K.P., Dose effects of an antiosteoporosis herbal formula - a preclinical investigation using a rat model, Phytother Res, 22, 2, pp. 267-273, (2008)
[3]  
Zhang Y., Lai W.P., Leung P.C., Wu C.F., Yao X.S., Wong M.S., Effects of Fructus Ligustri Lucidi extract on bone turnover and calcium balance in ovariectomized rats, Biol Pharm Bull, 29, 2, pp. 291-296, (2006)
[4]  
Zhang Y., Leung P.C., Che C.T., Chow H.K., Wu C.F., Wong M.S., Improvement of bone properties and enhancement of mineralization by ethanol extract of Fructus Ligustri Lucidi, Br J Nutr, 99, 3, pp. 494-502, (2008)
[5]  
Prockop D.J., Marrow stromal cells as stem cells for nonhematopoietic tissues, Science, 276, 5309, pp. 71-74, (1997)
[6]  
Nuttall M.E., Gimble J.M., Controlling the balance between osteoblastogenesis and adipogenesis and the consequent therapeutic implications, Curr Opin Pharmacol, 4, 3, pp. 290-294, (2004)
[7]  
Fu L., Tang T., Miao Y., Zhang S., Qu Z., Dai K., Stimulation of osteogenic differentiation and inhibition of adipogenic differentiation in bone marrow stromal cells by alendronate via ERK and JNK activation, Bone, 43, 1, pp. 40-47, (2008)
[8]  
Chinese Pharmacopoeia, (2010)
[9]  
Nordin B.E., Calcium and osteoporosis, Nutrition, 13, 4-8, pp. 664-686, (1997)
[10]  
Dobson K.R., Reading L., Haberey M., Marine X., Scutt A., Centrifugal isolation of bone marrow from bone: an improved method for the recovery and quantitation of bone marrow osteoprogenitor cells from rat tibiae and femurae, Calcif Tissue Int, 65, 5, pp. 411-413, (1999)
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