Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk

被引：0

作者：

Juan Ignacio García

Holden V. Kelley

Johanna Meléndez

Rosa Alejandra Alvarez de León

Alejandra Castillo

Sabeen Sidiki

Kizil A. Yusoof

Elizabete Nunes

Cesar López Téllez

Carlos Rodolfo Mejía-Villatoro

Janet Ikeda

Alberto L. García-Basteiro

Shu-Hua Wang

Jordi B. Torrelles

机构：

[1] Universitat Autònoma de Barcelona,PhD Programme in Methodology of Biomedical Research and Public Health, Department of Paediatrics, Obstetrics and Gynaecology, Preventive Medicine, and Public Health

[2] Centro de Investigação em Saude de Manhiça (CISM),Hospital Central de Maputo

[3] Texas Biomedical Research Institute,undefined

[4] Unidad de Atención Integral del VIH e Infecciones Crónicas del Hospital Roosevelt “Dr. Carlos Rodolfo Mejía Villatoro”,undefined

[5] Ciudad de Guatemala,undefined

[6] Sección de Microbiología,undefined

[7] Departamento de Laboratorios Clínicos,undefined

[8] Hospital Roosevelt,undefined

[9] Ciudad de Guatemala,undefined

[10] Clinica de Atención Integral Dr. Isaac Cohen Alcahe,undefined

[11] Hospital de Especialidad Dr. Robles,undefined

[12] Department of Microbial Infection and Immunity,undefined

[13] College of Medicine (COM),undefined

[14] The Ohio State University (OSU),undefined

[15] Division of Pulmonology,undefined

[16] Investigación,undefined

[17] Desarrollo,undefined

[18] y Educación Integral,undefined

[19] ISGlobal,undefined

[20] Hospital Clínic - Universitat de Barcelona,undefined

[21] Amsterdam Institute for Global Health and Development (AIGHD),undefined

[22] Department of Internal Medicine,undefined

[23] Division of Infectious Diseases,undefined

[24] COM,undefined

[25] OSU,undefined

来源：

Scientific Reports | / 9卷

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摘要：

Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with α-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.

引用

共 55 条

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