Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H (vol 110, pg 1377, 2017)

被引：0

作者：

Casillo, Angela ^{[1
]}

Stahle, Jonas ^{[2
]}

Parrilli, Ermenegilda ^{[1
]}

Sannino, Filomena ^{[1
]}

Mitchell, Daniel E. ^{[3
,4
]}

Pieretti, Giuseppina ^{[1
]}

Gibson, Matthew I. ^{[3
,4
]}

Marino, Gennaro ^{[1
]}

Lanzetta, Rosa ^{[1
]}

Parrilli, Michelangelo ^{[5
]}

Widmalm, Goran ^{[2
]}

Tutino, Maria L. ^{[1
]}

Corsaro, Maria M. ^{[1
]}

机构：

[1] Univ Naples Federico II, Complesso Univ Monte S Angelo, Dept Chem Sci, Via Cintia 4, I-80126 Naples, Italy

[2] Stockholm Univ, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden

[3] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[4] Univ Warwick, Warwick Med Sch, Coventry CV4 7AL, W Midlands, England

[5] Univ Naples Federico II, Complesso Univ Monte S Angelo, Dept Biol, Via Cintia 4, I-80126 Naples, Italy

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2017年 / 110卷 / 11期

基金：

欧洲研究理事会;

关键词：

Anti-freeze activity; Cold adaptation; Extracellular polysaccharides; NMR; Psychrophile;

D O I：

10.1007/s10482-017-0900-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: -> 4)-beta-d-GlcpNAcA-(1 -> 3)-beta-d-QuipNAc4NAc-(1 -> 3)-beta-d-GalpNAc-(1 ->. The 3D model, generated in accordance with H-1,H-1-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 A degrees C, this molecule displays only a low ice recrystallization inhibition activity.

引用

页码：1389 / 1389

页数：1