Electrochemical immunosensor for the carcinoembryonic antigen based on a nanocomposite consisting of reduced graphene oxide, gold nanoparticles and poly(indole-6-carboxylic acid)

被引：0
作者：
Dan Zhao
Yang Wang
Guangming Nie
机构：
[1] Qingdao University of Science and Technology,Key Laboratory of Sensor Analysis of Tumor Markers, Ministry of Education, College of Chemistry and Molecular Engineering
来源：
Microchimica Acta | 2016年 / 183卷
关键词：
Electroanalysis; Differential pulse voltammetry; Ionic liquid; Layer-by-layer assembly; Nanomaterial; Cancer diagnosis; Indole-6-carboxylic acid;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We describe a label-free electrochemical immunosensor for the carcinoembryonic antigen (CEA). It is based on a nanocomposite consisting of electrochemically reduced graphene oxide, gold nanoparticles (AuNPs), and poly(indole-6-carboxylic acid). Coupled to nanoparticle-amplification techniques and modified with ionic liquid (IL), this immunoassay shows high sensitivity and good selectivity for CEA. At the best working voltage of 0.95 V (vs. Ag/AgCl), the lower detection limit is 0.02 ng·mL−1, and the response to CEA is linear in the range from 0.02 to 90 ng·mL−1. The method was applied to the determination of CEA in spiked serum samples and gave recoveries in the range from 98.5 % to 102 %.
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页码：2925 / 2932
页数：7
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