A sensitive multiplex PCR protocol for simultaneous detection of chicken, duck, and pork in beef samples

被引：0
作者：
Panzhu Qin
Wei Qu
Jianguo Xu
Dongqing Qiao
Li Yao
Feng Xue
Wei Chen
机构：
[1] Hefei University of Technology,School of Food and Biological Engineering, Engineering Research Center of Bio
[2] Hefei University of Technology,Process, MOE
[3] Nanjing Agricultural University,School of Biological and Medical Engineering
来源：
Journal of Food Science and Technology | 2019年 / 56卷
关键词：
Multiplex PCR; Identification; Chicken; Duck; Pork; Beef; Meat adulteration; Sensitivity; Specificity;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A rapid and sensitive multiplex PCR assay was developed for simultaneous identification of the adulteration ingredients of chicken, duck and pork in beef. Specific primers for the mitochondrial genes of Cyt b, CO III, ATPase subunit 8/6 and Cyt b of chicken, duck, pork, and beef, respectively, were adopted in the assay. DNA exaction from meat samples was carried out by using magnetic nanoparticles as rapid separation substrates. The multiplex PCR assay showed that the limit of detection was 0.05% for each species. Moreover, the multiplex PCR specifically identified five beef samples adulterated with pork and one beef samples adulterated with chicken among the 35 commercial samples examined, indicating the practicability of this multiplex PCR method for identifying adulterated ingredients of chicken, duck, and pork in commercial beef products.
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页码：1266 / 1274
页数：8
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