Simple method for DNA extraction from pancreatic juice for PCR amplification assays

被引:0
作者
Petra Müller
Ralf Jesnowski
Stefan Liebe
Arndt Rolfs
Matthias Löhr
机构
[1] University of Rostock,Division of Gastroenterology, Department of Medicine
[2] University of Rostock,Department of Neurology
来源
International Journal of Pancreatology | 1999年 / 25卷
关键词
PCR; pancreatic juice; DNA; ras; DNA preparation;
D O I
暂无
中图分类号
学科分类号
摘要
Conclusion: Preparation of DNA from pancreatic juice for subsequent polymerase chain reaction (PCR) is difficult, but manageable. The protocol presented offers a simple and fast solution. This method might be applicable to other complicated samples, such as saliva, wound secretions, or stool washings.
引用
收藏
页码:39 / 43
页数:4
相关论文
共 54 条
[1]  
Löhr M(1992)Application of the polymerase chain reaction in gastrointestinal endoscopy Endoscopy 24 779-781
[2]  
Brenner DA(1993)k-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization Am J Pathol 143 545-554
[3]  
Rooney JF(1994)Detection of point mutations in the k-ras oncogene at codon 12 in pure pancreatic juice for diagnosis of pancreatic carcinoma Cancer 73 1589-1594
[4]  
Nelson JA(1995)Restriktionsenzym-Mismatch-Polymerase-Kettenreaktion zum Nachweis von ki-ras-Onkogen -Mutationen beim Pankreaskarzinom Dtsch Med Wchnschr 120 826-830
[5]  
Hruban RH(1976)Assessment of ERCP and pure pancreatic juice cytology in patients with pancreatic disease Gut 17 14-21
[6]  
van Offershaus GJ(1994)Cytologic examination of pancreatic juice for differential diagnosis of benign and malignant mucin-producing tumors of the pancreas Cancer 74 826-833
[7]  
Allison DC(1997)Tissue homogenization as a key step extracting RNA from human and rat pancreatic tissue Biotechniques 22 408-412
[8]  
Kondo H(1990)Detection of cytomegalovirus nucleic acid sequences in pancreas in type 2 diabetes Lancet 336 644-648
[9]  
Sugano K(1996)Welche Bedeutung haben ras- und p53-Mutationen im Pankreassekret bei Patienten mit chronischer Pankreatitis? Z Gastroenterol 34 554-3606
[10]  
Fukayama N(1989)Modification of enzymatically amplified DNA for the detection of point mutations Nucleic Acid Res 17 3606-254