Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity

被引：0
作者：
Tiago Vieira Sousa
Eveline Teixeira Caixeta
Emilly Ruas Alkimim
Antonio Carlos Baião de Oliveira
Antonio Alves Pereira
Laércio Zambolim
Ney Sussumu Sakiyama
机构：
[1] Universidade Federal de Viçosa,Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), BioCafé
[2] Universidade Federal de Viçosa,Empresa Brasileira de Pesquisa Agropecuária
[3] Empresa de Pesquisa Agropecuária de Minas Gerais, Embrapa Café, BIOAGRO, BioCafé
[4] Empresa de Pesquisa Agropecuária de Minas Gerais,Embrapa Café
[5] Universidade Federal de Viçosa,Departamento de Fitopatologia
[6] Universidade Federal de Viçosa,Departamento de Fitotecnia
来源：
Euphytica | 2017年 / 213卷
关键词：
Fingerprinting; Molecular profiling; Genetic diversity; Cultivar identification; Protection of plant cultivars; Cultivars register; SSR marker;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes using morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this collection, and explore genetic kinships. Thirty-four C. arabica cultivars/progenies, which belong to the Brazilian Cultivar Trial, were analyzed using 31 microsatellite markers. Markers with weak bands were removed, and of the remaining, 74.07% were polymorphic and revealed 47 alleles. The obtained molecular profiles revealed segregation between and within cultivars/progenies, and genetic variability was observed between all the cultivars/progenies. Sixteen markers were selected for dendrogram construction and for fingerprinting analysis of the cultivars. The ability of these markers to detect varietal mixture and analyze diversity between and within cultivars was also discussed in detail. The results demonstrated the effectiveness of markers in distinguishing related genotypes from those with similar phenotypic traits. This biotechnological tool will assist breeders in DUS testing of cultivars.
引用
收藏
相关论文
共 159 条
  • [1] Anthony F(2001)Genetic diversity of wild coffee ( Euphytica 118 53-65
  • [2] Bertrand B(2002) L.) using molecular markers Theor Appl Genet 104 894-900
  • [3] Quiros O(2015)The origin of cultivated Food Secur 7 303-321
  • [4] Anthony F(2012) L. varieties revealed by AFLP and SSR markers Mol Plant Breed 3 11-25
  • [5] Combes C(2011)The coffee rust crises in Colombia and Central America (2008–2013): impacts, plausible causes and proposed solutions BMC Genom 12 304-264
  • [6] Astorga C(2010)Assessment of genetic diversity and population structure in a selected germplasm collection of 292 jute genotypes by microsatellite (SSR) markers Euphytica 173 255-282
  • [7] Avelino J(2009)Identification of SNP and SSR markers in eggplant using RAD tag sequencing Pesqui Agropecuária Bras 44 276-34
  • [8] Cristancho M(1981)Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene Novas variedades mais produtivas. Agric Hoje 6 32-196
  • [9] Georgiou S(2016)Herança da resistência do Híbrido de Timor UFV 443-03 à ferrugem-do-cafeeiro Tree Genet Genomes 12 7-1180
  • [10] Banerjee S(2005)DNA fingerprinting of oil camellia cultivars with SSR markers Euphytica 142 169-276
12345678910