Statistical optimization of process parameters for exopolysaccharide production by Aureobasidium pullulans using sweet potato based medium

被引：0
作者：
Sethuraman Padmanaban
Nagarajan Balaji
Chandrasekaran Muthukumaran
Krishnamurthi Tamilarasan
机构：
[1] Madha Engineering College,Department of Biotechnology
[2] Government College of Technology,Department of Industrial Biotechnology
[3] SRM University,Department of Chemical Engineering, School of Bioengineering
来源：
3 Biotech | 2015年 / 5卷
关键词：
Sweet potato; Exopolysaccharide; Response surface methodology; Central composite design;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Statistical experimental designs were applied to optimize the fermentation medium for exopolysaccharide (EPS) production. Plackett–Burman design was applied to identify the significance of seven medium variables, in which sweet potato and yeast extract were found to be the significant variables for EPS production. Central composite design was applied to evaluate the optimum condition of the selected variables. Maximum EPS production of 9.3 g/L was obtained with the predicted optimal level of sweet potato 10 %, yeast extract 0.75 %, 5.5 pH, and time 100 h. The determined (R2) value was 0.97, indicating a good fitted model for EPS production. Results of this study showed that sweet potato can be utilized as a low-cost effective substrate for pullulan production in submerged fermentation.
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页码：1067 / 1073
页数：6
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