Purification and Characterization of Pepsin-Solubilized Collagen from Skin of Sea Cucumber Holothuria parva

被引：0
作者：
Nasim Adibzadeh
Saeed Aminzadeh
Shahla Jamili
Ali Asghar Karkhane
Naser Farrokhi
机构：
[1] National Institute of Genetic Engineering and Biotechnology (NIGEB),Department of Animal and Marine Biotechnology
[2] Islamic Azad University,Department of Marine Biology Marine Science and Technology, Science and research Branch
[3] Shahrood University of Technology,Department of Agronomy and Plant Breeding, Faculty of Agriculture
来源：
Applied Biochemistry and Biotechnology | 2014年 / 173卷
关键词：
Sea cucumber; Pepsin-solubilized collagen; Isolation and characterization;
D O I：
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学科分类号：
摘要：
Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum transition (Tm) and denaturation temperature (Td) of PSC were determined to be 46.94 and 32.5 °C, respectively. The amino acid composition analysis revealed that glycine, proline, alanine, and hydroxyproline were the abundant amino acids available in extracted PSC. The results showed that the isolated collagen from H. parva has some similar characteristics to previously reported collagens used in food and pharmaceutical industries.
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页码：143 / 154
页数：11
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