The growth kinetic, differentiation properties, karyotyping, and characterization of adipose tissue-derived stem cells in hamster

被引：0

作者：

Mehrabani D. ^{[1
]}

Rabiee M. ^{[1
]}

Tamadon A. ^{[1
]}

Zare S. ^{[1
]}

Razeghian Jahromi I. ^{[2
]}

Dianatpour M. ^{[1
,3
]}

Khodabandeh Z. ^{[1
]}

机构：

[1] Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz

[2] Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz

[3] Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz

来源：

Comparative Clinical Pathology | 2016年 / 25卷 / 5期

关键词：

Adipose tissue; Differentiation; Growth kinetics; Mesenchymal stem cells; RT-PCR;

D O I：

10.1007/s00580-016-2300-7

中图分类号：

学科分类号：

摘要：

Multilineage properties of mesenchymal stem cells (MSCs) cause their contribution in repair of different tissue. Adipose tissue is considered a good source of MSCs because of its abundance and easy access. The aims of this study were to characterize and determine the differentiation properties and growth kinetics of adipose tissue-derived stem cells (AT-SCs) from Syrian hamster. Fat tissue was isolated from the abdominal and pelvic regions of the hamster and by using type I collagenase, and cells were isolated and cultured. AT-SCs were characterized by morphology, cell surface markers, and adipogenic and osteogenic differentiations. AT-SCs were transferred to 24 well plates, and the growth kinetic was assessed by evaluation of the population doubling time (PDT). To show the chromosome stability of the AT-SCs, karyotyping was done. Hamsters’ AT-SCs showed fibroblast like morphology and expressed CD73 and CD29 but not CD45. AT-SCs could be differentiated to adipocytes and osteoblasts verified by Alizarin Red and Oil Red O staining methods. The PDT of the passages 2 and 3 of the AT-SCs were 51.6 and 80.6 h, respectively. The karyotyping was normal until passage 4. Hamsters’ AT-SCs showed mesenchymal properties denoting to these cells as a good source for the cell transplantation purposes in experimental and preclinical studies in hamster as an animal model. © 2016, Springer-Verlag London.

引用

页码：1017 / 1022

页数：5

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