Oestrogen deficiency modulates particle-induced osteolysis

被引：32
作者：
Nich C. [1 ]
Langlois J. [1 ]
Marchadier A. [2 ,3 ]
Vidal C. [3 ]
Cohen-Solal M. [4 ]
Petite H. [1 ]
Hamadouche M. [1 ]
机构：
[1] Laboratoire de Bioingénierie et Biomécanique Ostéo-articulaires, Faculté de Médecine Paris 7-Denis Diderot, 75010 Paris, 10, avenue de Verdun
[2] Useful Progress, 75008 Paris, 23, rue d'Anjou
[3] Institut Pasteur and INSERM U747, Laboratory of Stem cells, Signaling and Prions, Université Paris 5-René Descartes, 75270 Paris Cedex 06
[4] INSERM U606, Faculté de Médecine Paris 7-Denis Diderot, 75010 Paris, 10, avenue de Verdun
来源：
Arthritis Research & Therapy | / 13卷 / 3期
关键词：
Normal Control Group; Oestrogen Deficiency; Bone Thickness; Osteoclast Number; Calvarial Bone;
D O I：
10.1186/ar3381
中图分类号：
学科分类号：
摘要：
Introduction: Postmenopausal osteoporosis may modulate bone response to wear debris. In this article, we evaluate the influence of oestrogen deficiency on experimental particle-induced osteolysis.Methods: Polyethylene (PE) particles were implanted onto the calvaria of normal controls, sham-ovariectomized (OVX), OVX mice and OVX mice supplemented with oestrogen (OVX+E). After 14 days, seven skulls per group were analyzed using a high-resolution micro-computed tomography (micro-CT) and histomorphometry, and for tartrate-specific alkaline phosphatase. Five calvariae per group were cultured for the assay of IL-1β, IL-6, TNF-α and receptor activator of the nuclear factor κB (RANKL) secretion using quantitative ELISA. Serum IL-6 concentrations were obtained. The expression of RANKL and osteoprotegerin (OPG) mRNA were evaluated using real-time PCR.Results: As assessed by μCT and by histomorphometry, PE particles induced extensive bone resorption and an intense inflammatory reaction in normal controls, sham-OVX and OVX+E mice, but not in the OVX mice group. In normal controls, sham-OVX and OVX+E mice, PE particles induced an increase in serum IL-6, in TNF-α and RANKL local concentrations, and resulted in a significant increase in RANKL/OPG messenger RNA (mRNA) ratio. Conversely, these parameters remained unchanged in OVX mice after PE implantation.Conclusions: Oestrogen privation in the osteolysis murine model ultimately attenuated osteolytic response to PE particles, suggesting a protective effect. This paradoxical phenomenon was associated with a down-regulation of pro-resorptive cytokines. It is hypothesized that excessive inflammatory response was controlled, illustrated by the absence of increase of serum IL-6 in OVX mice after PE implantation. © 2011 Nich et al.; licensee BioMed Central Ltd.
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