High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology

被引：0
作者：
Ya. A. Lomakin
A. N. Kaminskaya
A. V. Stepanov
A. A. Shmidt
A. G. Gabibov
A. A. Belogurov
机构：
[1] M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry,
[2] Russian Academy of Sciences,undefined
[3] Kazan (Volga region) Federal University,undefined
[4] M. V. Lomonosov Moscow State University,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2019年 / 167卷
关键词：
M13K07 bacteriophage; B-cell receptor; phage display; follicular lymphoma; flow cytofluorometry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.
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页码：446 / 451
页数：5
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  • [1] Amanna IJ(2006)Quantitation of rare memory B cell populations by two independent and complementary approaches J. Immunol. Methods. 317 175-185
  • [2] Slifka MK(2008)Identification and characterization of nonapeptide targeting a human B cell lymphoma, Raji Int. Immunopharmacol. 8 852-858
  • [3] Choi JH(2018)Follicular lymphoma: 2018 update on diagnosis and management Am. J. Hematol. 93 296-305
  • [4] Lee WK(2006)A hybrid vector for ligand-directed tumor targeting and molecular imaging Cell. 125 385-398
  • [5] Han SH(2018)Risk factors for Burkitt lymphoma: a nested case-control study in the UK Clinical Practice Research Datalink Br. J. Haematol. 181 505-514
  • [6] Ha S(2018)Pathogenesis of follicular lymphoma Best Pract. Res. Clin. Haematol. 31 2-14
  • [7] Ahn SM(2017)Follicular lymphomas and their transformation: Past and current research Expert Rev. Hematol. 10 515-524
  • [8] Kang JS(2012)Non-Hodgkin lymphoma Lancet. 380 848-857
  • [9] Choi YJ(2017)Pathways towards indolent B-cell lymphoma — Etiology and therapeutic strategies Blood Rev. 31 426-435
  • [10] Yun CH(2004)Monoclonal antibody therapy of B cell lymphoma Expert Opin. Biol. Ther. 4 375-385
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