Genome-wide evolutionary analysis of TKL_CTR1-DRK-2 gene family and functional characterization reveals that TaCTR1 positively regulates flowering time in wheat

被引:1
作者
Su, Peisen S. [1 ]
Li, Jingyu [1 ]
Zang, Dongtian [1 ]
Wang, Zhiyu [1 ]
Wu, Yangyang [1 ]
Chi, Shatong [1 ]
Sun, Fanting [1 ]
Niu, Yufei [1 ]
Hua, Xuewen [1 ]
Yan, Jun [2 ]
Ge, Wenyang [3 ]
机构
[1] Liaocheng Univ, Coll Agron, Liaocheng 252059, Peoples R China
[2] Shandong Agr Univ, Coll Informat Sci & Engn, Key Lab Huang Huai Hai Smart Agr Technol, Minist Agr & Rural Affairs, Tai An 271018, Shandong, Peoples R China
[3] Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China
关键词
Serine/threonine-protein kinases; Flowering; Transcriptomics analysis; TaCTR1; PROTEIN-KINASE; ETHYLENE; ARABIDOPSIS; CLASSIFICATION; CTR1; DIVERGENCE; PHYLOGENY; CONSTANS; TOMATO;
D O I
10.1186/s12864-024-10383-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background Flowering time has an important effect on regional adaptation and yields for crops. The tyrosine kinase-like (TKL) gene family is widely existed and participates in many biological processes in plants. Furthermore, only few TKLs have been characterized functions in controlling flowering time in wheat. Results Here, we report that TaCTR1, a tyrosine kinase-like (TKL) gene, regulates flowering time in wheat. Based on identification and evolutionary analysis of TKL_CTR1-DRK-2 subfamily in 15 plants, we proposed an evolutionary model for TaCTR1, suggesting that occurrence of some exon fusion events during evolution. The overexpression of TaCTR1 caused early flowering time in transgenic lines. Transcriptomics analysis enabled identification of mass differential expression genes including plant hormone (ET, ABA, IAA, BR) signaling, flavonoid biosynthesis, phenolamides and antioxidant, and flowering-related genes in TaCTR1 overexpression transgenic lines compared with WT plants. qRT-PCR results showed that the expression levels of ethylene (ET) signal-related genes (ETR, EIN, ERF) and flowering-related genes (FT, PPD1, CO, PRR, PHY) were altered in TaCTR1-overexpressing wheat compared with WT plants. Metabonomics analysis showed that flavonoid contents were altered. Conclusions Thus, the results show that TaCTR1 plays a positive role in controlling flowering time by activating various signaling pathways and regulating flowering-related genes, and will provide new insights on the mechanisms of wheat flowering regulation.
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页数:17
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