Emulsified isoflurane postconditioning produces cardioprotection against myocardial ischemia–reperfusion injury in rats

被引：0
作者：
Zhao-Yang Hu
Geoffrey W. Abbott
Ya-Dong Fang
Yue-Sheng Huang
Jin Liu
机构：
[1] Sichuan University,Department of Anesthesiology and Translational Neuroscience Center, West China Hospital
[2] School of Medicine,Department of Pharmacology, Physiology and Biophysics
[3] University of California,State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital
[4] Irvine,undefined
[5] Third Military Medical University,undefined
来源：
The Journal of Physiological Sciences | 2013年 / 63卷
关键词：
Emulsified isoflurane; Ischemia and reperfusion injury; Cardioprotection;
D O I：
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学科分类号：
摘要：
Emulsified isoflurane (EIso) preconditioning can induce cardioprotection. We investigated whether EIso application after ischemia protects hearts against reperfusion injury and whether it is mediated by the inhibition of apoptosis. Rats were subjected to 30-min coronary occlusion followed by 180-min reperfusion. At the onset of reperfusion, rats were intravenously administered saline (sham, control group), 30 % intralipid (IL group) or 2 ml kg−1 EIso (EIso group) for 30 min. After reperfusion, infarct sizes, myocardial apoptosis and expression of Bcl-2, Bax and caspase-3 proteins were determined. Hemodynamic parameters were not different among groups. Compared with control and intralipid group, EIso limited infarct size, inhibited apoptosis, increased the expression of Bcl-2, decreased the expression of Bax, cleaved caspase-3, and enhanced Bcl-2/Bax ratio. EIso protects hearts against reperfusion injury when administered at the onset of reperfusion, which may be mediated by the inhibition of apoptosis via modulation of the expression of pro- and anti-apoptotic proteins.
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页码：251 / 261
页数：10
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