Apoptotic cell death is an important cause of neuronal injury in experimental Venezuelan equine encephalitis virus infection of mice

被引：0

作者：

A. C. Jackson

John P. Rossiter

机构：

[1] Department of Medicine,

[2] Queen’s University,undefined

[3] 78 Barrie Street Kingston,undefined

[4] Ontario,undefined

[5] Canada K7L 3J7 Tel.: 1-613-545-6425; Fax: 1-613-545-6591; e-mail: jacksona@post.queensu.ca,undefined

[6] Department of Microbiology and Immunology,undefined

[7] Queen’s University,undefined

[8] Kingston,undefined

[9] Ontario,undefined

[10] Canada,undefined

[11] Department of Pathology,undefined

[12] Queen’s University,undefined

[13] Kingston,undefined

[14] Ontario,undefined

[15] Canada,undefined

来源：

Acta Neuropathologica | 1997年 / 93卷

关键词：

Key words Apoptosis; Arbovirus; Encephalitis; Pathogenesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Mice develop a fatal encephalomyelitis after infection with the Trinidad donkey strain of Venezuelan equine encephalitis (VEE) virus. Adult mice were inoculated intraperitoneally with VEE virus and the brains were examined at different time points. Morphological changes were assessed by histological staining. VEE virus antigen was detected with immunoperoxidase staining, and DNA fragmentation was evaluated in situ using the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) method. VEE antigen was found in many areas of the brain and it was prominent in neurons. There were mild associated inflammatory changes. DNA fragmentation was demonstrated in many of these areas using TUNEL. In areas with TUNEL staining, morphological neuronal changes ranged from nuclear chromatin condensations to nuclear and cellular fragmentation, which are characteristic of apoptosis. There is strong morphological and biochemical evidence of apoptotic cell death in this experimental model of VEE virus infection.

引用

页码：349 / 353

页数：4