Cardiac optogenetics: a decade of enlightenment

被引：0
作者：
Emilia Entcheva
Matthew W. Kay
机构：
[1] George Washington University,Department of Biomedical Engineering
来源：
Nature Reviews Cardiology | 2021年 / 18卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The electromechanical function of the heart involves complex, coordinated activity over time and space. Life-threatening cardiac arrhythmias arise from asynchrony in these space–time events; therefore, therapies for prevention and treatment require fundamental understanding and the ability to visualize, perturb and control cardiac activity. Optogenetics combines optical and molecular biology (genetic) approaches for light-enabled sensing and actuation of electrical activity with unprecedented spatiotemporal resolution and parallelism. The year 2020 marks a decade of developments in cardiac optogenetics since this technology was adopted from neuroscience and applied to the heart. In this Review, we appraise a decade of advances that define near-term (immediate) translation based on all-optical electrophysiology, including high-throughput screening, cardiotoxicity testing and personalized medicine assays, and long-term (aspirational) prospects for clinical translation of cardiac optogenetics, including new optical therapies for rhythm control. The main translational opportunities and challenges for optogenetics to be fully embraced in cardiology are also discussed.
引用
收藏
页码：349 / 367
页数：18
相关论文
共 457 条
[11]  
Salama G(2005)Next-generation optical technologies for illuminating genetically targeted brain circuits Nat. Neurosci. 8 17816-17821
[12]  
Tallini YN(2005)Millisecond-timescale, genetically targeted optical control of neural activity Proc. Natl Acad. Sci. USA 102 2279-2284
[13]  
Roell W(2005)Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin Curr. Biol. 15 13940-13945
[14]  
Chi NC(2003)Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses Proc. Natl Acad. Sci. USA 100 2395-2398
[15]  
Deisseroth K(2002)Channelrhodopsin-2, a directly light-gated cation-selective membrane channel Science 296 395-399
[16]  
Boyden ES(2009)Channelrhodopsin-1: a light-gated proton channel in green algae Science 326 15-22
[17]  
Zhang F(2002)The optogenetic catechism Neuron 33 1352-1357
[18]  
Bamberg E(2003)Selective photostimulation of genetically charged neurons Proc. Natl Acad. Sci. USA 100 1386-1389
[19]  
Nagel G(2012)Photochemical gating of heterologous ion channels: remote control over genetically designated populations of neurons Annu. Int. Conf. Proc. IEEE Eng. Med. Biol. Soc. 2012 H1179-H1191
[20]  
Deisseroth K(2013)Cardiac optogenetics Am. J. Physiol. Heart. Circ. Physiol. 304 294-304
12345678910