Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10

被引：0
作者：
Angela Dittmer
Kristina Hohlfeld
Jana Lützkendorf
Lutz P. Müller
Jürgen Dittmer
机构：
[1] Universität Halle,Klinik für Gynäkologie
[2] Universität Halle,Zentrum für Innere Medizin, Klinik für Innere Medizin IV
来源：
Cellular and Molecular Life Sciences | 2009年 / 66卷
关键词：
MSC; Spheroids; Breast cancer; Migration; ADAM 10; E-cadherin;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Mesenchymal stem cells (MSCs) have been shown to communicate with tumor cells. We analyzed the effect of human MSCs (hMSCs) on breast cancer cells in three-dimensional cultures. By using GFP expression and immunohistochemistry, we show that hMSCs invade 3D breast cancer cell aggregates. hMSCs caused breast cancer spheroids to become disorganized which was accompanied by a disruption of cell–cell adhesion, E-cadherin cleavage, and nuclear translocation of E-cadherin, but not by epithelial/mesenchymal transition or by an increase in ERK1/2 activity. In addition, hMSCs enhanced the motility of breast cancer cells. Inhibition of ADAM10 (a disintegrin and metalloprotease 10), known to cleave E-cadherin, prevented both hMSC-mediated E-cadherin cleavage and enhanced migration. Our data suggest that hMSCs interfere with cell–cell adhesion and enhance migration of breast cancer cells by activating ADAM10.
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页码：3053 / 3065
页数：12
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