Induction of RNA interference in dendritic cells

被引：0
作者：
Mu Li
Hua Qian
Thomas E. Ichim
Wei-Wen Ge
Igor A. Popov
Katarzyna Rycerz
John Neu
David White
Robert Zhong
Wei-Ping Min
机构：
[1] The University of Western Ontario,Department of Surgery, Microbiology and Immunology
[2] London Health Science Centre,Multi
[3] Lawson Health Research Institute,Organ Transplant Program
[4] The JP Robarts Research Institute,Immunology and Transplantation
来源：
Immunologic Research | 2004年 / 30卷
关键词：
Dendritic cells; Gene silencing; RNA interference; siRNA; Cytokines; Gene therapy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Dendritic cells (DC) reside at the center of the immunological universe, possessing the ability both to stimulate and inhibit various types of responses. Tolerogenic/regulatory DC with therapeutic properties can be generated through various means of manipulations in vitro and in vivo. Here we describe several attractive strategies for manipulation of DC using the novel technique of RNA interference (RNAi). Additionally, we overview some of our data regarding yet undescribed characteristics of RNAi in DC such as specific transfection strategies, persistence of gene silencing, and multi-gene silencing. The advantages of using RNAi for DC genetic manipulation gives rise to the promise of generating tailor-made DC that can be used effectively to treat a variety of immunologically mediated diseases.
引用
收藏
页码：215 / 230
页数：15
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