Pitfalls and Their Remedies in Time-Resolved Fluorescence Spectroscopy and Microscopy

被引：0
|
作者：
Martin vandeVen
Marcel Ameloot
Bernard Valeur
Noël Boens
机构：
[1] Transnationale Universiteit Limburg,Biomedisch Onderzoeksinstituut, Limburgs Universitair Centrum, School of Life Sciences
[2] CNAM,CNRS UMR 8531, Laboratoire de Chimie générale
[3] ENS-Cachan,CNRS UMR 8531, Laboratoire PPSM
[4] Katholieke Universiteit Leuven,Department of Chemistry
来源：
Journal of Fluorescence | 2005年 / 15卷
关键词：
Time-resolved fluorescence spectroscopy; time-resolved fluorescence microscopy; artifacts; pulse fluorometry; phase-modulation fluorometry; FLIM; single-photon timing; sample preparation; optics; electronics; data analysis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Time-resolved fluorescence spectroscopy and microscopy in both time and frequency domains provide very useful and accurate information on dynamic processes. Good quality data are essential in obtaining reliable parameter estimates. Distortions of the fluorescence response due to artifacts may have disastrous consequences. We provide here a concise overview of potential difficulties encountered under daily laboratory circumstances in the use of time- and frequency-domain equipment as well as practical remedies against common error conditions, elucidated with several graphs to aid the researcher in visual inspection and quality-control of collected data. A range of artifacts due to sample preparation or to optical and electronic pitfalls are discussed, as are remedies against them. Also recommended data analysis strategies are described.
引用
收藏
页码：377 / 413
页数：36
相关论文
共 50 条
  • [41] Detection of high-risk atherosclerotic lesions by time-resolved fluorescence spectroscopy based on the Laguerre deconvolution technique
    Jo, J. A.
    Fang, Q.
    Papaioannou, T.
    Qiao, J. H.
    Fishbein, M. C.
    Beseth, B.
    Dorafshar, A. H.
    Reil, T.
    Baker, D.
    Freischlag, J.
    Marcu, L.
    PHOTONIC THERAPEUTICS AND DIAGNOSTICS II, 2006, 6078
  • [42] Time-resolved fluorescence microscopy to study biological related applications using sol-gel derived and cellular media
    Toury, Marion
    Chandler, Lin
    Allison, Archie
    Campbell, David
    McLoskey, David
    Holmes-Smith, A. Sheila
    Hungerford, Graham
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI, 2011, 7903
  • [43] Fluorescence lifetime imaging microscopy and time-resolved anisotropy of nanomaterial-induced changes to red blood cell membranes
    Sydor, Matthew J.
    Anderson, Donald S.
    Steele, Harmen B. B.
    Ross, J. B. Alexander
    Holian, Andrij
    METHODS AND APPLICATIONS IN FLUORESCENCE, 2021, 9 (03):
  • [44] Estimation of crude oil grade using time-resolved fluorescence spectra
    Hegazi, E
    Hamdan, A
    TALANTA, 2002, 56 (06) : 989 - 995
  • [45] Remote fingerprinting of crude oil using time-resolved fluorescence spectra
    Hegazi, E
    Hamdan, A
    Mastromarino, J
    ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING, 2005, 30 (1B) : 3 - 12
  • [46] Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum
    Adams, Alexandra C.
    Kufcsak, Andras
    Ehrlich, Katjana
    Dhaliwal, Kevin
    Seth, Sohan
    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2023, 70 (08) : 2395 - 2403
  • [47] New methods for time-resolved fluorescence spectroscopy data analysis based on the Laguerre expansion technique - Applications in tissue diagnosis
    Jo, J. A.
    Marcu, L.
    Fang, Q.
    Papaioannou, T.
    Qiao, J. H.
    Fishbein, M. C.
    Beseth, B.
    Dorafshar, A. H.
    Reil, T.
    Baker, D.
    Freischlag, J.
    METHODS OF INFORMATION IN MEDICINE, 2007, 46 (02) : 206 - 211
  • [48] Application of Time-Resolved Fluorescence Microscopy for Enhancing the Selectivity of Fluorogenic Dyes of the Arylidene-Imidazolone Series toward the Endoplasmic Reticulum
    Gilvanov, A. R.
    Smirnov, A. Yu.
    Krasnova, S. A.
    Solovyev, I. D.
    Savitsky, A. P.
    Bogdanova, Yu. A.
    Baranov, M. S.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2024, 50 (05) : 1896 - 1903
  • [49] Time-resolved and spectrally-resolved 5D multiphoton microscopy for analysis and nanoprocessing of materials
    König, K
    Riemann, I
    Schuck, H
    Sauer, D
    Velten, T
    Le Harzic, R
    Photon Processing in Microelectronics and Photonics IV, 2005, 5713 : 552 - 559
  • [50] Steady-state and time-resolved fluorescence studies of stripped Borage oil
    Smyk, Bogdan
    Amarowicz, Ryszard
    Szabelski, Mariusz
    Gryczynski, Ignacy
    Gryczynski, Zygmunt
    ANALYTICA CHIMICA ACTA, 2009, 646 (1-2) : 85 - 89
12345