Pitfalls and Their Remedies in Time-Resolved Fluorescence Spectroscopy and Microscopy

被引：0
作者：
Martin vandeVen
Marcel Ameloot
Bernard Valeur
Noël Boens
机构：
[1] Transnationale Universiteit Limburg,Biomedisch Onderzoeksinstituut, Limburgs Universitair Centrum, School of Life Sciences
[2] CNAM,CNRS UMR 8531, Laboratoire de Chimie générale
[3] ENS-Cachan,CNRS UMR 8531, Laboratoire PPSM
[4] Katholieke Universiteit Leuven,Department of Chemistry
来源：
Journal of Fluorescence | 2005年 / 15卷
关键词：
Time-resolved fluorescence spectroscopy; time-resolved fluorescence microscopy; artifacts; pulse fluorometry; phase-modulation fluorometry; FLIM; single-photon timing; sample preparation; optics; electronics; data analysis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Time-resolved fluorescence spectroscopy and microscopy in both time and frequency domains provide very useful and accurate information on dynamic processes. Good quality data are essential in obtaining reliable parameter estimates. Distortions of the fluorescence response due to artifacts may have disastrous consequences. We provide here a concise overview of potential difficulties encountered under daily laboratory circumstances in the use of time- and frequency-domain equipment as well as practical remedies against common error conditions, elucidated with several graphs to aid the researcher in visual inspection and quality-control of collected data. A range of artifacts due to sample preparation or to optical and electronic pitfalls are discussed, as are remedies against them. Also recommended data analysis strategies are described.
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页码：377 / 413
页数：36
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