Gene expression profile of endometrial carcinoma cells exposed to di-(2-ethylhexyl) phthalate

被引:0
作者
Hyun-Hee Cho
Mee Song
Jae Chun Ryu
机构
[1] Catholic University Medical College,Department of Ob/Gyn, Seoul St. Mary’s Hospital
[2] Korea Institute of Science & Technology,Cellular and molecular toxicology laboratory, Center for Integrated Risk Research
来源
Molecular & Cellular Toxicology | 2013年 / 9卷
关键词
DEHP; Endometrial cancer cell; Microarray;
D O I
暂无
中图分类号
学科分类号
摘要
To evaluation of the contribution of DEHP (di-(2-ethylhexyl) phthalate) to the pathophysiology of endometriosis, ECC-1 (human endometrial cancer) cell lines were subjected to DEHP for 48 h with 50 μM DEHP and total RNA was extracted. Quantified spectro-photometrically, and RNA quality was verified via agarose-gel electrophoresis. Gene expression changes were analyzed using oligonucleotide microarray hybridization by comparing the treated and control groups (two-fold change, P<0.05). Three independent samples were analyzed. Data indicated that 522 genes were up-regulated and 379 were down-regulated. These genes were classified according to the GO terms of biological processes and the KEGG pathway. Among biologic processes, regulation of cell death, lipid metabolism, gluconeogenesis, response to hormone stimulus, and regulation of cell differentiation were positively associated with up-regulated genes. Mitosis, meiosis, DNA damage check point, anatomical structure development, reproductive development, and regulation of protein ubiquitination were significantly associated with down-regulated genes. In the KEGG pathway analysis, the MAPK, VEGF, p53, Erbb, mTOR, and phosphatidylinositol signaling pathways were positively associated with up-regulated genes. The cell cycle, oocyte meiosis, spliceosome, and pyrimidine metabolism were positively associated with downregulated genes. In conclusion, After DEHP treatment, some genes associated with cell growth, cell differentiation, meiosis, migration, apoptosis, and the pathophysiology of endometriosis were up regulated.
引用
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页码:113 / 120
页数:7
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