Expression of RUNX2 and Osterix in Rat Mesenchymal Stem Cells during Culturing in Osteogenic-Conditioned Medium

被引：0
作者：
L. A. Pokrovskaya
S. V. Nadezhdin
E. V. Zubareva
Yu. E. Burda
E. S. Gnezdyukova
机构：
[1] National Research Tomsk State University,Research Laboratory “Cellular, Assisted Reproductive, and DNA Technologies”
[2] Belgorod State National Research University,undefined
[3] Innovation Centre “Biruch — New Technologies”,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2020年 / 169卷
关键词：
mesenchymal stem cells; osteoinduction; differentiation; osteogenic-conditioned medium;
D O I：
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学科分类号：
摘要：
We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.
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页码：571 / 575
页数：4
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