Interaction between the helicase domain of the tobacco mosaic virus replicase and a tobacco arginine decarboxylase

被引：5

作者：

Shimizu T. ^{[1
,2
]}

Yamaji Y. ^{[1
]}

Ogasawara Y. ^{[1
]}

Hamada K. ^{[1
]}

Sakurai K. ^{[1
]}

Kobayashi T. ^{[1
]}

Watanabe T. ^{[1
]}

Hibi T. ^{[1
]}

机构：

[1] Laboratory of Plant Pathology, Grad. Sch. of Agric. and Life Sci., University of Tokyo, Bunkyo-ku, Tokyo 113-8657

[2] Natl. Agricultural Research Center, Ibaraki

来源：

Journal of General Plant Pathology | 2004年 / 70卷 / 6期

基金：

日本学术振兴会;

关键词：

Arginine decarboxylase; Nicotiana tabacum; Replicase; Tobacco mosaic virus; Yeast two-hybrid screening;

D O I：

10.1007/s10327-004-0139-2

中图分类号：

学科分类号：

摘要：

In a yeast two-hybrid screening test for tobacco proteins that interact with TMV replicase using the helicase (H) domain as bait, a cDNA clone was selected that encodes a polyamine biosynthetic enzyme, arginine decarboxylase (ADC). In yeast cells, the C-terminal internal region of ADC interacted with the H domain. This observation was confirmed in vitro by far-Western blotting. Inhibition of the binding between the H domain and the IRnHEL (I region and N-terminus of helicase domain) region by ADC using a yeast three-hybrid assay suggested possible interference of the heterodimerization of 126K and 183K by ADC.

引用

页码：353 / 358

页数：5

共 23 条

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