Estrogen directly down-regulates the bone-resorbing activity of mature osteoclasts through nuclear estrogen receptor α

被引：0
作者：
Hiroshi Mano
Yoshiyuki Hakeda
Masayoshi Kumegawa
机构：
[1] Tokyo University of Agriculture,Department of Bioscience, Faculty of Applied Bioscience
[2] Meikai University School of Dentistry,Department of Oral Anatomy
[3] Meikai University School of Dentistry,Department of Oral Anatomy
来源：
Cytotechnology | 2001年 / 35卷
关键词：
estrogen; estrogen receptor; gene expression; osteoclast; osteoporosis; pit assay;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The decrease in estrogen level that follows the onset ofmenopause causes rapid bone loss, resulting in osteoporosis.However, the mechanism remains unclear, especially concerningthe regulation of bone-resorbing osteoclasts. Here we analyzedthe function of estrogen and its receptor in matureosteoclasts. We found that estrogen directly inhibitedbone-resorption by purified rabbit mature-osteoclasts.Moreover, using a RT-PCR technique, we report that nuclearestrogen receptor (ER) α but not ERβ is expressed in mature osteoclasts. The antisense oligodeoxynucleotide for ERα inhibited the reductionin osteoclastic bone-resorbing activity caused by estrogen. We conclude that in part estrogen directly inhibits the bone-resorbing activity of mature osteoclasts through the ERα.
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页码：17 / 23
页数：6
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