Keth-seq for transcriptome-wide RNA structure mapping

被引：0
|
作者：
Xiaocheng Weng
Jing Gong
Yi Chen
Tong Wu
Fang Wang
Shixi Yang
Yushu Yuan
Guanzheng Luo
Kai Chen
Lulu Hu
Honghui Ma
Pingluan Wang
Qiangfeng Cliff Zhang
Xiang Zhou
Chuan He
机构：
[1] Institute for Biophysical Dynamics,Department of Chemistry, Department of Biochemistry and Molecular Biology
[2] Howard Hughes Medical Institute,College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education
[3] The University of Chicago,MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua
[4] Wuhan University,Peking Joint Center for Life Sciences, School of Life Sciences
[5] Tsinghua University,undefined
[6] Wuhan University School of Pharmaceutical Sciences,undefined
来源：
Nature Chemical Biology | 2020年 / 16卷
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摘要：
RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.
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页码：489 / 492
页数：3
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