Interaction of Cutibacterium ( formerly Propionibacterium) acnes with bone cells: a step toward understanding bone and joint infection development

被引：0
作者：
Guillaume Ghislain Aubin
Marc Baud’huin
Jean-Philippe Lavigne
Régis Brion
François Gouin
Didier Lepelletier
Cédric Jacqueline
Dominique Heymann
Karim Asehnoune
Stéphane Corvec
机构：
[1] EA3826,Department of Microbiology
[2] Laboratory of Clinical and Experimental Therapeutics of Infections,Department of Oncology and Metabolism
[3] IRS2,undefined
[4] 22 Bd Benoni-Goullin,undefined
[5] University of Nantes,undefined
[6] Bacteriology and Hygiene Unit,undefined
[7] CHU NANTES,undefined
[8] INSERM,undefined
[9] UMR 957,undefined
[10] Pathophysiology of Bone Resorption Laboratory and Therapy of Primary Bone Tumors,undefined
[11] Medicine School,undefined
[12] University of Nantes,undefined
[13] CHU NANTES,undefined
[14] Hôtel Dieu,undefined
[15] INSERM,undefined
[16] U1047,undefined
[17] University of Montpellier,undefined
[18] Caremeau University Hospital,undefined
[19] Clinique chirurgicale orthopédique et traumatique,undefined
[20] CHU NANTES,undefined
[21] Medical School,undefined
[22] University of Sheffield,undefined
[23] CRCINA,undefined
[24] INSERM,undefined
[25] Université d’Angers,undefined
[26] Université de Nantes,undefined
来源：
Scientific Reports | / 7卷
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摘要：
Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by live C. acnes. The whole genome sequence of six isolates of this collection was analyzed. CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C. acnes strains (p ≤ 0.05). The CC18 C. acnes ATCC6919 isolate could survive intracellularly for at least 96 hours. C. acnes significantly decreased the resorption ability of osteoclasts with a major impact by the CC36 strain (p ≤ 0.05). Genome analysis revealed 27 genes possibly linked to these phenotypic behaviors. We showed a direct impact of C. acnes on bone cells, providing new explanations about the development of C. acnes foreign-body infections.
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[1]  
Deschaseaux F(2009)Mechanisms of bone repair and regeneration Trends Mol Med 15 417-429
[2]  
Sensébé L(2014): an update on its role in the pathogenesis of acne J Eur Acad Dermatol Venereol 28 271-278
[3]  
Heymann D(2014), an emerging pathogen: from acne to implant-infections, from phylotype to resistance Med Mal Infect 44 241-250
[4]  
Beylot C(2014): from Commensal to Opportunistic Biofilm-Associated Implant Pathogen Clin. Microbiol. Rev. 27 419-440
[5]  
Aubin GG(2013): An Underestimated Pathogen in Implant-Associated Infections Biomed Res Int 2013 804391-934
[6]  
Portillo ME(2012)Epidemiology and new developments in the diagnosis of prosthetic joint infection Int J Artif Organs 35 923-e25
[7]  
Trampuz A(2013)Diagnosis and management of prosthetic joint infection: clinical practice guidelines by the Infectious Diseases Society of America Clin. Infect. Dis. 56 e1-1046
[8]  
Corvec S(2008) spp. in prosthetic joint infections: a diagnostic challenge Arch Orthop Trauma Surg 128 1039-3678
[9]  
Achermann Y(2013)Prosthesis failure within 2 years of implantation is highly predictive of infection Clin. Orthop. Relat. Res 471 3672-425
[10]  
Goldstein EJC(2010)Improving diagnostic criteria for Scand. J. Infect. Dis. 42 421-1165
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