Determination of cortisol in guinea pig urine by high performance thin-layer chromatography, high performance liquid chromatography, and thin-layer chromatography/radioimmunoassay

被引：0
作者：
M. Fenske
机构：
[1] University of Bayreuth,Department of Animal Physiology
[2] NWI,undefined
来源：
Chromatographia | 1997年 / 44卷
关键词：
Thin layer chromatography; Column liquid chromatography; Radioimmunoassay; Cortisol; Guinea pig; Urine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In order to collect urinary samples from unrestrained guinea pigs, animals were kept in their familiar home cages with wood shavings for bedding. Cortisol was removed from shavings by a simple washing step, and an attempt was made to measure its concentrations by high performance thin-layer chromatography (HPTLC), high performance liquid chromatography (HPLC), or thin layer chromatography/radioimmunoassay (TLC-RIA). After intramuscular administration of 25 mg cortisol, cortisol excretion increased from about 20–30 μg/day to 400–500 μg/day (HPTLC: 531 μg/day, HPLC: 493 μg/day; TLC-RIA: 394 μg/day). Similarly, the treatment of the animals with 20 IU ACTH resulted in an augmented cortisol excretion, with mean values of 294 μg/day (HPTLC), 256 μg/day (HPLC) and 143 μg/day (TLC-RIA), respectively.
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页码：50 / 54
页数：4
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