In vitro cultivation and differentiation of fetal liver stem cells from mice

被引：0
作者：
Ren Qing FENG
Li Ying DU
Zhen Quan GUO
机构：
[1] College of Life Sciences,
[2] Peking University,undefined
来源：
Cell Research | 2005年 / 15卷
关键词：
Hepatic stem/progenitor cell; diabetes; β-cell; dithizone staining; immunocytochemistry;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
During embryonic development, pluripotent endoderm tissue in the developing foregut may adopt pancreatic fate or hepatic fate depending on the activation of key developmental regulators. Transdifferentiation occurs between hepatocytes and pancreatic cells under specific conditions. Hepatocytes and pancreatic cells have the common endodermal progenitor cells. In this study we isolated hepatic stem/progenitor cells from embryonic day (ED) 12-14 Kun-Ming mice with fluorescence-activated cell sorting (FACS). The cells were cultured under specific conditions. The cultured cells deploy dithizone staining and immunocytochemical staining at the 15th, 30th and 40th day after isolation. The results indicated the presence of insulin-producing cells. When the insulin-producing cells were transplanted into alloxan-induced diabetic mice, the nonfasting blood glucose level was reduced. These results suggested that fetal liver stem/progenitor cells could be converted into insulin-producing cells under specific culture conditions. Fetal liver stem/progenitor cells could become the potential source of insulin-producing cells for successful cell transplantation therapy strategies of diabetes.
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页码：401 / 405
页数：4
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  • [1] Mathis DL(2001)beta-Cell death during progression to diabetes Nature 414 792-8
  • [2] Vence L(2002)Pancreatic stem cells J Pathol 197 519-26
  • [3] Benoist C(2000)Human islet transplantation: lessons from 13 autologous and 13 allogeneic transplantations Transplantation 69 1115-23
  • [4] Bonner-Weir S(2003)Pancreatic-hepatic switches Mech Dev 120 99-106
  • [5] Sharma A(2003)Organogenesis: making pancreas from liver Curr Biol 13 R96-8
  • [6] Oberholzer J(2002)Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver J Cell Biol 156 173-84
  • [7] Triponez F(2003)Experimental conversion of liver to pancreas Curr Biol 13 105-15
  • [8] Mage R(2002)Combined expression of pancreatic duodenal homeobox 1 and islet factor 1 induces immature enterocytes to produce insulin Diabetes 51 1398-408
  • [9] Grompe M(2003)Glucagon-like peptide 1 (1-37) converts intestinal epithelial cells into insulin-producing cells Proc Natl Acad Sci U S A 100 5034-9
  • [10] McLin VA(2002) trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells Proc Natl Acad Sci U S A 99 8078-83
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