Functional Analysis of the Anthocyanin-Associated Glutathione S-Transferase Gene StGST1 in Potato

被引：0
作者：
Zhonghua Zhang
Huiling Zhang
Tengfei Liu
Yanan Zhao
XiJuan Zhao
Ju Liu
Juping Zhang
机构：
[1] Henan University of Science and Technology,College of Horticulture and Plant Protection
[2] Huazhong Agricultural University,Key Laboratory of Horticultural Plant Biology, Ministry of Education
[3] Huazhong Agricultural University,Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs
[4] Hunan Agricultural University,College of Horticulture
来源：
Potato Research | 2023年 / 66卷
关键词：
Anthocyanin; Potato; Transcription activation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Anthocyanins are biosynthesized on the cytoplasmic surface of the endoplasmic reticulum and then transported to the vacuole for storage. Glutathione S-transferases (GSTs) are involved in the transport of anthocyanins. However, the regulatory mechanism of GSTs in potato is unclear. In this study, the glutathione S-transferase gene StGST1 was isolated from potato and characterized. Expression analysis revealed that StGST1 transcript levels were higher in pigmented tubers than in white tubers. Overexpression of StGST1 promoted anthocyanin accumulation in the tuber. Transient luciferase analysis revealed that the promoter of StGST1 was activated by the transcription factors StAN2 and StAN11. These results indicated that StGST1 promotes anthocyanin accumulation in potato tubers. These findings have important implications for improvement of anthocyanin accumulation in pigmented potato tubers.
引用
收藏
页码：215 / 230
页数：15
相关论文
共 188 条
  • [1] Brown CR(2005)Antioxidants in potato Am J Potato Res 82 163-172
  • [2] El-Sharkawy I(2015)Transcriptome analysis of an apple ( J Exp Bot 66 7359-7376
  • [3] Liang D(2013) × Plant Cell 25 1840-1854
  • [4] Xu K(2009)) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation Plant Physiol 150 402-415
  • [5] Francisco RM(2004)ABCC1, an ATP binding cassette protein from grape berry, transports anthocyanidin 3-O-glucosides Plant Cell 16 1812-1826
  • [6] Regalado A(2016)Grapevine MATE-type proteins act as vacuolar H Plant Cell Rep 35 831-843
  • [7] Ageorges A(2019)-dependent acylated anthocyanin transporters Hortic Res 6 40-57
  • [8] Burla BJ(2021)A multidrug resistance-associated protein involved in anthocyanin transport in J Plant Physiol 257 153353-642
  • [9] Bassin B(2009) is an anthocyanin-related Theor Appl Genet 120 45-114
  • [10] Eisenach C(2012) gene in J Plant Physiol 169 636-372
12345678910