Reconstruction of non-uniformly sampled five-dimensional NMR spectra by signal separation algorithm

被引：0
作者：
Krzysztof Kosiński
Jan Stanek
Michał J. Górka
Szymon Żerko
Wiktor Koźmiński
机构：
[1] University of Warsaw,Faculty of Chemistry, Biological and Chemical Research Centre
[2] University of Warsaw,Faculty of Physics, Division of Biophysics, Institute of Experimental Physics
来源：
Journal of Biomolecular NMR | 2017年 / 68卷
关键词：
High-dimensional NMR; 5D; Non-uniform sampling; Spectral reconstruction; Signal separation algorithm; Intrinsically disordered proteins; Resonance assignment;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method for five-dimensional spectral reconstruction of non-uniformly sampled NMR data sets is proposed. It is derived from the previously published signal separation algorithm, with major alterations to avoid unfeasible processing of an entire five-dimensional spectrum. The proposed method allows credible reconstruction of spectra from as little as a few hundred data points and enables sensitive resonance detection in experiments with a high dynamic range of peak intensities. The efficiency of the method is demonstrated on two high-resolution spectra for rapid sequential assignment of intrinsically disordered proteins, namely 5D HN(CA)CONH and 5D (HACA)CON(CO)CONH.
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页码：129 / 138
页数：9
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