Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

被引：0
作者：
Makiko Yamashita
Yoshinori Katakura
Yoshihiro Aiba
Shin-ei Matsumoto
Kazuko Morihara
Kiichiro Teruya
Akira Ichikawa
Sanetaka Shirahata
机构：
[1] Kyushu University,Graduate School of Systems Life Sciences
[2] Kyushu University,Department of Genetic Resources Technology, Faculty of Agriculture
[3] Kyoto Institute of Technology,Department of Applied Biology
来源：
Cytotechnology | 2007年 / 55卷
关键词：
Antibody production; Interleukin-10; CD11c; dendritic cells; In vitro immunization; Peripheral blood mononuclear cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
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页码：71 / 77
页数：6
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