Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells

被引：0
|
作者：
Makiko Yamashita
Yoshinori Katakura
Yoshihiro Aiba
Shin-ei Matsumoto
Kazuko Morihara
Kiichiro Teruya
Akira Ichikawa
Sanetaka Shirahata
机构：
[1] Kyushu University,Graduate School of Systems Life Sciences
[2] Kyushu University,Department of Genetic Resources Technology, Faculty of Agriculture
[3] Kyoto Institute of Technology,Department of Applied Biology
来源：
Cytotechnology | 2007年 / 55卷
关键词：
Antibody production; Interleukin-10; CD11c; dendritic cells; In vitro immunization; Peripheral blood mononuclear cells;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Previously, we have established an in vitro immunization method to induce antigen-specific antibody-producing B cells. In the present study, we have attempted to clarify the mechanisms that regulate antibody production by in vitro immunized peripheral blood mononuclear cells (PBMC). Freshly isolated PBMC did not induce antibody production following in vitro immunization, but expressed the interleukin (IL)-10 gene. On the other hand, PBMC pretreated with l-leucyl-l-leucine methyl ester (LLME) induced antibody production, but did not express the IL-10 gene. IL-10 induced functional impairment of CD4+ Th cells and CD11c+ DC, resulting in the suppression of antibody production by in vitro immunized PBMC.
引用
收藏
页码：71 / 77
页数：6
相关论文
共 50 条
  • [1] Involvement of IL-10 in the suppression of antibody production by in vitro immunized peripheral blood mononuclear cells
    Yamashita, Makiko
    Katakura, Yoshinori
    Aiba, Yoshihiro
    Matsumoto, Shin-ei
    Morihara, Kazuko
    Teruya, Kiichiro
    Ichikawa, Akira
    Shirahata, Sanetaka
    CYTOTECHNOLOGY, 2007, 55 (2-3) : 71 - 77
  • [2] Mild hypothermia inhibits IL-10 production in peripheral blood mononuclear cells
    Matsui, T
    Ishikawa, T
    Takeuchi, H
    Tsukahara, M
    Maekawa, T
    ACTA ANAESTHESIOLOGICA SCANDINAVICA, 2004, 48 (02) : 205 - 210
  • [3] IL-10 synthesis and secretion by peripheral blood mononuclear cells in haemodialysis patients
    Brunet, P
    Capo, C
    Dellacasagrande, J
    Thirion, X
    Mege, JL
    Berland, Y
    NEPHROLOGY DIALYSIS TRANSPLANTATION, 1998, 13 (07) : 1745 - 1751
  • [4] Anti-peptide antibody production elicited by in vitro immunization of human peripheral blood mononuclear cells
    Tamura, Takashi
    Tomimatsu, Kosuke
    Katakura, Yoshinori
    Yamashita, Makiko
    Matsumoto, Shin-ei
    Aiba, Yoshihiro
    Jung, Yeon Suk
    Abe, Yoshiichi
    Fujiki, Tsukasa
    Teruya, Kiichiro
    Shirahata, Sanetaka
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71 (12) : 2871 - 2875
  • [5] IL-10 augments antibody production in in vitro immunized lymphocytes by inducing a Th2-type response and B cell maturation
    Xu, QH
    Katakura, Y
    Yamashita, M
    Fang, S
    Tamura, T
    Matsumoto, S
    Aiba, Y
    Teruya, K
    Osada, K
    Nishikawa, R
    Shirahata, S
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2004, 68 (11) : 2279 - 2284
  • [6] A three base pair gene variation within the distal 5′-flanking region of the interleukin-10 (IL-10) gene is related to the in vitro IL-10 production capacity of lipopolysaccharide-stimulated peripheral blood mononuclear cells
    Rieth, H
    Mörmann, M
    Assohou-Luty, CA
    Roupelieva, M
    Kremsner, PG
    Kube, D
    EUROPEAN CYTOKINE NETWORK, 2004, 15 (02) : 153 - 158
  • [7] In vitro immune responses of human peripheral blood mononuclear cells to silk fibroin: IL-10 stimulated anti-inflammatory and hypoallergenic properties
    Chancheewa, Bussabong
    Buranapraditkun, Supranee
    Laomeephol, Chavee
    Rerknimitr, Pawinee
    Kanokpanont, Sorada
    Damrongsakkul, Siriporn
    Klaewsongkram, Jettanong
    MATERIALS TODAY COMMUNICATIONS, 2020, 24
  • [8] Age-related impaired proliferation of peripheral blood mononuclear cells is associated with an increase in both IL-10 and IL-12
    Castle, SC
    Uyemura, K
    Crawford, W
    Wong, W
    Klaustermeyer, WB
    Makinodan, T
    EXPERIMENTAL GERONTOLOGY, 1999, 34 (02) : 243 - 252
  • [9] Differential expression of interferon-gamma, IL-4 and IL-10 in peripheral blood mononuclear cells during early pregnancy of the bovine
    Yang, Ling
    Wang, Yongxiang
    Li, Shujing
    Zhu, Meixia
    He, Kaili
    Yao, Xiaolei
    Zhang, Leying
    REPRODUCTIVE BIOLOGY, 2018, 18 (03) : 312 - 315
  • [10] Decreased mRNA expression of TNF-α and IL-10 in non-stimulated peripheral blood mononuclear cells in myasthenia gravis
    Huang, WX
    Huang, P
    Fredrikson, S
    Pirskanen, R
    Hillert, J
    EUROPEAN JOURNAL OF NEUROLOGY, 2000, 7 (02) : 195 - 202
12345