1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein

被引：0
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作者：
Jiajing Liu
Zhenming Du
Clayton D. Albracht
Roshni O. Naidu
Kenneth V. Mills
Chunyu Wang
机构：
[1] Center for Biotechnology and Interdisciplinary Studies,Biology Department
[2] Rensselaer Polytechnic Institute,Department of Chemistry
[3] College of the Holy Cross,undefined
来源：
Biomolecular NMR Assignments | 2011年 / 5卷
关键词：
Intein; NMR; Assignment; Hyperthermophile; DNA polymerase II;
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摘要：
Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the 15N, 13C, and 1H chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.
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