1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein

被引：0
作者：
Jiajing Liu
Zhenming Du
Clayton D. Albracht
Roshni O. Naidu
Kenneth V. Mills
Chunyu Wang
机构：
[1] Center for Biotechnology and Interdisciplinary Studies,Biology Department
[2] Rensselaer Polytechnic Institute,Department of Chemistry
[3] College of the Holy Cross,undefined
来源：
Biomolecular NMR Assignments | 2011年 / 5卷
关键词：
Intein; NMR; Assignment; Hyperthermophile; DNA polymerase II;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the 15N, 13C, and 1H chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.
引用
收藏
相关论文
共 43 条
[1]  
Amitai G(2004)Protein splicing of inteins with atypical glutamine and aspartate C-terminal residues J Biol Chem 279 3121-3131
[2]  
Dassa B(2009)Highly conserved histidine plays a dual catalytic role in protein splicing: a pKa shift mechanism J Am Chem Soc 131 11581-11589
[3]  
Pietrokovski S(2010)Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins J Mol Biol 400 755-767
[4]  
Du Z(2010)Inteins, valuable genetic elements in molecular biology and biotechnology Appl Microbiol Biotechnol 87 479-489
[5]  
Shemella PT(2010)Branched intermediate formation stimulates peptide bond cleavage in protein splicing Nat Chem Biol 6 527-533
[6]  
Liu Y(2007)NMR structure of a KlbA intein precursor from Protein Sci 16 1316-1328
[7]  
McCallum SA(2004)Protein splicing of a J Biol Chem 279 20685-20691
[8]  
Pereira B(2009) intein with a C-terminal glutamine FEBS Lett 583 1451-1456
[9]  
Nayak SK(2000)Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification Annu Rev Biochem 69 447-496
[10]  
Belfort G(2006)Protein splicing and related forms of protein autoprocessing Chem Record 6 183-193
12345