Evaluation of the mecA femB Duplex Polymerase Chain Reaction for Detection of Methicillin-Resistant Staphylococcus aureus

被引：0
|
作者：
D. Jonas
H. Grundmann
D. Hartung
F. D. Daschner
K.J. Towner
机构：
[1] Institute of Environmental Medicine and Hospital Epidemiology,
[2] University Hospital Freiburg,undefined
[3] Hugstetter Strasse 55,undefined
[4] D-79106 Freiburg,undefined
[5] Germany,undefined
[6] Department of Microbiology & Public Health Laboratory Service Laboratory,undefined
[7] University Hospital,undefined
[8] Queens Medical Centre,undefined
[9] Nottingham NG7 2UH,undefined
[10] UK,undefined
来源：
European Journal of Clinical Microbiology and Infectious Diseases | 1999年 / 18卷
关键词：
Polymerase Chain Reaction; Staphylococcus Aureus; Susceptibility Testing; Accurate Method; Reaction Test;
D O I：
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中图分类号：
学科分类号：
摘要：
 This study systematically evaluated a recently described duplex polymerase chain reaction test for methicillin-resistant Staphylococcus aureus with 25 different German epidemic strains of methicillin-resistant Staphylococcus aureus and 66 staphylococci other than methicillin-resistant Staphylococcus aureus, including 17 different coagulase-negative staphylococcal species and subspecies, that were either oxacillin susceptible or oxacillin resistant. The results were compared with those of conventional cultural identification and susceptibility testing. Of the 91 isolates tested, all 25 confirmed strains of methicillin-resistant Staphylococcus aureus were identified correctly. None of the remaining strains of methicillin-susceptible Staphylococcus aureus was misidentified as methicillin-resistant Staphylococcus aureus. It was concluded that the duplex polymerase chain reaction appears to offer a time-saving and accurate method of detection of methicillin-resistant Staphylococcus aureus.
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页码：643 / 647
页数：4
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